In Vitro Study of Anti-cancer Properties of Egyptian Scorpion (Leiurus quinquestriatus) Venom on Triple Negative Human Breast Cancer Cell Line MDA‑ MB‑ 231 | ||||
| Bulletin of Faculty of Science, Zagazig University | ||||
| Article 11, Volume 2024, Issue 4, January 2025, Page 108-118 PDF (1.23 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfszu.2024.278885.1379 | ||||
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| Authors | ||||
Jihad El-Qassas  1; Mahmoud Abdelatti2; Nagwa El-Badri3
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| 1Department of zoology, Faculty of science, Zagazig university, Egypt | ||||
| 2Department of Zoology, Faculty of Science, Zagazig University, Zagazig 44519, Egypt. | ||||
| 3Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, 6th of October City, Giza 12578, Egypt | ||||
| Abstract | ||||
| Despite advances in therapy of breast cancer, it still remains a significant health concern. During the past recent years, various research studies have encompassed that novel therapeutic approaches are urgently needed. Scorpion venom has emerged as a promising anti-cancer agent. This is achieved primarily via scorpion venom-derived peptides revealed high and selective affinity for ionic channels over-expressed in many cancer cells. In particular, This study refer to the anti-cancer properties of L. quinquestriatus venom against human breast cancer cells MDA-MB-231. Cell viability was assessed using MTT assay. Apoptosis induction was evaluated using Annexin V/PI staining kit. Wound healing assay was employed to analyze cell migration. The MTT assay results demonstrated that L. quinquestriatus venom decreased the cell viability and had a selectively cytotoxicity against cancer cells, owing to its possible anti-cancer activity. The Annexin V-PI staining revealed a substantial increase in the percentage of apoptotic cells upon L. quinquestriatus venom treatment, and the wound healing assay showed a marked reduction by (~70%) in the migration ability after L. quinquestriatus venom treatment in comparison with non-treated cells. Significant decrease in human breast cancer cell viability (~ 60%) was observed after 48 hrs. of treatment with L. quinquestriatus venom compared with non-treated cells (P < 0.0001). These present findings provide evidence that L. quinquestriatus venom possesses potent anti-cancer properties and may be useful in the development of breast cancer treatment strategies. | ||||
| Keywords | ||||
| Leiurus quinquestriatus venom; Cell viability; Apoptosis; Cell migration; Human Breast Cancer Cell Line (MDA-MB-231) | ||||
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