Green tea extract supplementation optimizes sperm quality, DNA integrity, and antioxidant activity of cryopreserved buffalo bull (Bubalus bubalis) semen | ||||
| Kafrelsheikh Veterinary Medical Journal | ||||
| Volume 23, Issue 1, January 2025, Page 1-9 PDF (306.88 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/kvmj.2025.346040.1040 | ||||
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| Authors | ||||
Asmaa Salah Ghania  1; Alaa Elsayed Abdel-Ghaffar2; Gamal Abdel-raheem Sosa1; Magdy Ramadan Badr3; Mohsen AbdElhafeez Agag1; Mohammed Mahmoud M Kandeel4; Laila Esmaail Kortam5; Dina Mahdy Shokry3
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| 1Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt. | ||||
| 2Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Delta University for Science and Technology, Gamasa, Dakahlia, Egypt. | ||||
| 3Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 12556 Haram, Giza, Egypt | ||||
| 4Theriogenology Department, Faculty of Veterinary Medicine, Benha University, EGYPT | ||||
| 5Immunity Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), 12556 Haram, Giza, Egypt. | ||||
| Abstract | ||||
| Background and Aim: Freezing and thawing processes increase the susceptibility of spermatozoa to oxidative stress, which negatively impacts sperm cells functions and reduces its potential fertilizing capacity. The current study aimed to explore the ameliorative effects of various proportions of GTE on post-thawing buffalo bull spermiograms, DNA integrity, and antioxidant activities. Methods: Phytochemical analysis of GTE was done by high-performance liquid chromatography (HPLC). Seventy-two ejaculates were collected from fertile buffalo bulls weekly (n = 12) for 6 weeks, diluted in a tris-based extender supplemented with GTE (0%, 0.1%, 0.5%, 0.75%, 1.0%, and 2.0%) and assessed for sperm motilitiy characterestics, acrosome, plasma membrane and DNA integrities, as well as for the activities of antioxidant enzymes (superoxide dismutase, glutathione reduced, catalase, and total antioxidant activity) and lipid peroxidation (MDA). Results and conclusion: Phytochemical analysis of GTE by high-performance liquid chromatography (HPLC) indicated the presence of five high polyphenol content with high accounting for epigallocatechin-3 gallate and epicatechin. Computer-assisted sperm analysis (CASA) showed the highest total and progressive motility values, and wobble coefficient (WOB) was obtained with 0.75% GTE. At the same time, the distance of average path (DAP) was enhanced with 2.0% GTE compared to the control. Incorporation of 0.75% GTE significantly (P< 0.001) increased acrosome and plasma membrane integrities, and reduced the percentage of DNA-damage, tail length, and tail moment of frozen-thawed buffalo bull spermatozoa. Activities of antioxidant enzymes (superoxide dismutase, glutathione reduced, catalase, and total antioxidant activity) improved with 0.75% GTE, whereas lipid peroxidation (MDA) was reduced significantly (P˂ 0.001) with 0.5%, 0.75%, and 1.0% GTE compared to control. In conclusion, the addition of 0.75% GTE in buffalo bull semen diluted with a tris-based extender ameliorated the cryodamage influence on sperm parameters as well as reduced DNA damage with optimum antioxidant capacity. | ||||
| Keywords | ||||
| Keywords: Buffalo bull; Cryopreservation; Green tea; HPLC; Sperm kinetics | ||||
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