Biochemical Parameters in Milk Triggered by Bacterial Pathogens Causing Subclinical Mastitis | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 28 January 2025 PDF (808.58 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.340010.2542 | ||||
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| Authors | ||||
Hala Ragab Khalil Ali  1; Nagla F Koraney2; Samah F. Ali3; Esraa Gaber Hefny4; Mahmoud Arafa5; Momtaz A. Shahein 6
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| 1Bacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC), Dokki, Giza, Egypt. | ||||
| 2Bacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC), 12618 Dokki, Giza, Egypt. | ||||
| 3Bacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC), 12618 Dokki, Giza, Egypt | ||||
| 4Senior researcher Food Hygiene Department, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC), Dokki, Giaz12618,Egypt. | ||||
| 5Biochemistry and Toxicology Department, Animal Health Research Institute, Agriculture Research Center, Giza, Egypt; | ||||
| 6Department of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, Egypt | ||||
| Abstract | ||||
| Subclinical mastitis is characterized by inflammation in the udder without any signs of clinical illness. Udder inflammation is mainly associated with major alterations in biochemical milk components, which may be identified as indicators for pathogenic infections. To identify key biochemical changes in milk relevant to early detection of subclinical mastitis, milk samples were collected from 10 healthy cows and 50 cows diagnosed with subclinical mastitis based on the California Mastitis Test, followed by analysis of bacterial contents and biochemical components. The bacteriological analysis revealed that S. aureus was the main pathogen associated with subclinical cases (32%) either as a single infection or mixed with E. coli (4%) or Klebsiella spp,. (8%). Single bacterial infections by E. coli, Klebsiella spp., and Enterobacter spp. were detected in 7%, 7% and 3% of the subclinical mastitis cases, respectively. Biochemical composition analysis of bacteriologically positive milk samples versus healthy samples showed a significant decrease in important electrolytes (Ca, P, K, Mg) and total proteins, in addition to a significant increase in blood electrolytes (Na & Cl), and C-reactive proteins (CRP). The result showed no significant difference in all parameters except for CRP between single and mixed bacterial infections. Albumin concentrations showed no significant changes in all tested milk samples, while vitamin E & A concentrations showed variation among the tested milk samples. In conclusion, CRP as well as total protein, blood and important electrolytes, have potential as biomarkers for subclinical mastitis, suggesting that they could be integrated as practical tests to monitor dairy herd health and early detection of subclinical mastitis. | ||||
| Keywords | ||||
| S.aureus; subclinical mastitis; CRP; electrolytes; biochemical parameters | ||||
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