Enhancement of Bioactive Secondary Metabolites Productivity by Marine Streptomyces acrimycini MBS-HRS-EG (MYI1) using Blackett-Burman Design and Scaling up using 7.5 L Bioreactor with In vitro Antifungal, Antioxidant and Anticancer Assessment | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 29 January 2025 PDF (1.28 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.345456.2568 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ismail G. Said1; Mai Raslan2; Amr E. Ahmed3; Hassan A. El-Menoufy4; Hassan M Awad  5
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| 11Chemistry of Natural and Microbial Products Dept., Pharmaceutical Industries Div., National Research Centre, 33 EL Bohouth St., Dokki, 12622, Giza, Egypt. 2Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies | ||||
| 2Department of Biotechnology and Life Sciences, Faculty of Postgraduate studies for Advanced Sciences, Beni-Suef University | ||||
| 3Biotechnology and life Sciences department, Faculty of postgraduate studies for advanced Sciences, Beni-suef University | ||||
| 41Chemistry of Natural and Microbial Products Dept., Pharmaceutical Industries Div., National Research Centre, 33 EL Bohouth St., Dokki, 12622, Giza, Egypt. | ||||
| 5Chemistry of Natural and Microbial Products Department, National Research Centre | ||||
| Abstract | ||||
| Marine actinomycetes, especially Streptomyces sp., are known for producing diverse biologically active compounds like antifungal, antioxidant, and anticancer agents. This study aims to optimize the production of bioactive secondary metabolites by marine Streptomyces acrimycini strain MBS-HRS-EG (MYI1) ID: PP477812.1 through submerged fermentation. The study involved screening for antifungal and antioxidant activities using agar well diffusion and DPPH methods. A medium pre-screening identified the most effective medium, which was further optimized using a Plackett-Burman design (PBD) with eleven factors and fourteen runs. Statistical analysis showed significant models with a lack-of-fit of 0.212 and R2 above 0.9768 for all responses. MgSO4, sodium pyruvate, and (NH4)2SO4 were key bioactivity variables. The optimized conditions were tested in a 7.5 L bioreactor at different agitation speeds, showing higher productivity at 200 and 400 rpm. Cytotoxicity assessment revealed the efficacy of the bioactive compounds against various cancer cell lines, with IC50 values ranging from 75.8 to 108.5 µg/mL. | ||||
| Keywords | ||||
| Marine Streptomyces; Statistical optimization by Blackett-Burman design; Antifungal; Antioxidant and Anticancer; Scaling up with 7.5 L bioreactor | ||||
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