Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs
Elmasry, D., Said, D., El-Shaarawy, D., EL-Husseini, D., Adel, A., El-kanawaty, Z., Abo Hatab, E., Shahein, M. (2025). Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs. EKB Journal Management System, (), 1-10. doi: 10.21608/ejvs.2025.335949.2491
Dalia M.A. Elmasry; Dalia Said; Dina O. El-Shaarawy; Dalia M. EL-Husseini; Amany Adel; Zakaria R. El-kanawaty; Eman M. Abo Hatab; Momtaz A. Shahein. "Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs". EKB Journal Management System, , , 2025, 1-10. doi: 10.21608/ejvs.2025.335949.2491
Elmasry, D., Said, D., El-Shaarawy, D., EL-Husseini, D., Adel, A., El-kanawaty, Z., Abo Hatab, E., Shahein, M. (2025). 'Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs', EKB Journal Management System, (), pp. 1-10. doi: 10.21608/ejvs.2025.335949.2491
Elmasry, D., Said, D., El-Shaarawy, D., EL-Husseini, D., Adel, A., El-kanawaty, Z., Abo Hatab, E., Shahein, M. Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs. EKB Journal Management System, 2025; (): 1-10. doi: 10.21608/ejvs.2025.335949.2491

Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs

Egyptian Journal of Veterinary Sciences
Articles in Press, Corrected Proof, Available Online from 29 January 2025  XML PDF (1.11 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2025.335949.2491
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Authors
Dalia M.A. Elmasry email orcid 1; Dalia Saidorcid 2; Dina O. El-Shaarawyorcid 3; Dalia M. EL-Husseiniorcid 4; Amany Adelorcid 3; Zakaria R. El-kanawatyorcid 3; Eman M. Abo Hataborcid 5; Momtaz A. Shaheinorcid 6
17th nadi elsaid, Animal Health Research Institute, Agriculture Research Center
2Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt..
3Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt.
4Nanomaterial synthesis and Research Unit, Animal Health Research Institute, Agricultural Research Center (ARC), Giza, Egypt, Postal Code: 264.
5Virology research Dept., Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt
6Virology research Dept., Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt.
Abstract
This study investigates the antiviral potential of Propolis-pollen nanoemulsion  (PP-NE) against four major poultry viruses: H5N8 avian influenza (H5), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and infectious bursal disease virus (IBDV). PP-Ne were prepared using a phase titration approach and registered under Egyptian Academy of Scientific Research number EG/P/2024/653. The nanoparticles were characterized by LC-MS/MS and transmission electron microscopy (TEM), revealing a spherical shape with an average size of 13.34±2.102 nm. The cytotoxicity assessment using Vero cells and Sulforhodamine B (SRB) stain indicated an IC50 of 23.19 μg/mL. Phenolic compounds, including curcumadiol and methyl oleate, were identified as key components.To assess the antiviral efficacy, virus-nanoparticle mixtures were incubated at 37°C for 2, 4, 8, and 24 hours before inoculating specific pathogen-free embryonated chicken eggs (SPF-ECE). Viral activity was monitored using real-time quantitative reverse transcription PCR (qRT-PCR) tests. The results demonstrated significant inhibition of viral replication for all tested viruses.  H5N8 after 8 hours (p = 0.009), IBDV after 24 hours (p = 0.002192), NDV after 4 hours (p = 0.004106), and IBV showed complete inhibition after 24 hours (p = 0.000255) .These findings highlight the potential of PP-NE as an effective antiviral agent in veterinary medicine, warranting further research into their application for controlling viral infections in poultry.
Keywords
Propolis-pollen nanoemulsion (PP-NE); Avian influenza (H5N8); Newcastle disease virus (NDV); Infectious bronchitis virus (IBV); Infectious bursal disease virus (IBDV); Phase titration; LC-MS/MS
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