Highlight on Propolis-Pollen Nanoemulsion Effect on Some Avian Viruses on Chicken Embryo Eggs | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 29 January 2025 PDF (1.11 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.335949.2491 | ||
| Authors | ||
| Dalia M.A. Elmasry* 1; Dalia Said2; Dina O. El-Shaarawy3; Dalia M. EL-Husseini4; Amany Adel3; Zakaria R. El-kanawaty3; Eman M. Abo Hatab5; Momtaz A. Shahein6 | ||
| 17th nadi elsaid, Animal Health Research Institute, Agriculture Research Center | ||
| 2Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt.. | ||
| 3Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt. | ||
| 4Nanomaterial synthesis and Research Unit, Animal Health Research Institute, Agricultural Research Center (ARC), Giza, Egypt, Postal Code: 264. | ||
| 5Virology research Dept., Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt | ||
| 6Virology research Dept., Animal Health Research Institute, Agriculture Research Center P.O. Box 264-Dokki, Giza 12618, Egypt. | ||
| Abstract | ||
| This study investigates the antiviral potential of Propolis-pollen nanoemulsion (PP-NE) against four major poultry viruses: H5N8 avian influenza (H5), Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and infectious bursal disease virus (IBDV). PP-Ne were prepared using a phase titration approach and registered under Egyptian Academy of Scientific Research number EG/P/2024/653. The nanoparticles were characterized by LC-MS/MS and transmission electron microscopy (TEM), revealing a spherical shape with an average size of 13.34±2.102 nm. The cytotoxicity assessment using Vero cells and Sulforhodamine B (SRB) stain indicated an IC50 of 23.19 μg/mL. Phenolic compounds, including curcumadiol and methyl oleate, were identified as key components.To assess the antiviral efficacy, virus-nanoparticle mixtures were incubated at 37°C for 2, 4, 8, and 24 hours before inoculating specific pathogen-free embryonated chicken eggs (SPF-ECE). Viral activity was monitored using real-time quantitative reverse transcription PCR (qRT-PCR) tests. The results demonstrated significant inhibition of viral replication for all tested viruses. H5N8 after 8 hours (p = 0.009), IBDV after 24 hours (p = 0.002192), NDV after 4 hours (p = 0.004106), and IBV showed complete inhibition after 24 hours (p = 0.000255) .These findings highlight the potential of PP-NE as an effective antiviral agent in veterinary medicine, warranting further research into their application for controlling viral infections in poultry. | ||
| Keywords | ||
| Propolis-pollen nanoemulsion (PP-NE); Avian influenza (H5N8); Newcastle disease virus (NDV); Infectious bronchitis virus (IBV); Infectious bursal disease virus (IBDV); Phase titration; LC-MS/MS | ||
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