Production and properties of dextransucrase by free and immobilized cells of | ||||
| Egyptian Pharmaceutical Journal | ||||
| Volume 11, Issue 1, June 2012, Page 42-48 PDF (378.42 K) | ||||
| DOI: 10.7123/01.EPJ.0000415613.02268.4d | ||||
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| Authors | ||||
| Ahmed F. Abdel Fattah; Amal M. Hashem; Mona A. El-Refai; Hassan M. Gebreel | ||||
| Abstract | ||||
Six bacterial strains were tested for the production of dextransucrase. The highest dextransucrase activities were produced by local strain after 24 h in static culture conditions. Dextransucrase was induced using sucrose (10 g%) as a carbon source and corn steep liquor (3.5 ml%) as a nitrogen source. Fe favoured enzyme production. Cells of were immobilized using three supports, calcium alginate, agar and κ-carrageenan. Calcium alginate beads were able to produce dextransucrase for four runs with 100% activity. The optimum temperature for enzyme reaction ranged from 30 to 65πC at pH 5.4. In the absence of substrate, the enzyme retained 100% activity for 30 min at 40πC and 69% activity for 10 min at 60πC. | ||||
| Keywords | ||||
| Cell immobilization; dextransucrase properties | ||||
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