Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods | ||
| Egyptian Pharmaceutical Journal | ||
| Volume 12, Issue 2, July 2013, Pages 136-141 PDF (598.7 K) | ||
| DOI: 10.4103/1687-4315.124014 | ||
| Authors | ||
| Mohamed A. Zewail; Somya Abdel-Rahman; Ahmed M. Naglah; Ahmed M. Shalaby | ||
| Abstract | ||
| Background and objective This work embraced a systematic search for potentiated methodologies for peptide synthesis through a di-dimensional approach for convenient synthesis of the nonapeptide (B–B) of porcine insulin B-chain. Liquid-phase peptide synthesis (LPPS) and liquid–solid-phase peptide synthesis (modified SPPS) were used for the synthesis of this active part. Materials and methods A nonapeptide Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala-OH corresponding to B–B of porcine insulin B-chain was synthesized using different methods: LPPS and modified SPPS. Time consumption, yield and purity of all products using the different methods were compared with each other. Results and conclusion The results indicated that modified SPPS is advantageous, as it consumes less solvent per coupling and deprotection reaction, thereby reducing operating costs and solvent waste. Reducing side reactions result in racemization, cyclization or premature peptide formation. Modified SPPS produces peptides with yields and purity better than LPPS. Also, it can reduce the time of coupling and deprotection reactions. | ||
| Keywords | ||
| Amino acids; Insulin; Liquid phase; modified solid phase; Peptide Synthesis | ||
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