Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods
Zewail, M., Abdel-Rahman, S., Naglah, A., Shalaby, A. (2013). Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods. EKB Journal Management System, 12(2), 136-141. doi: 10.4103/1687-4315.124014
Mohamed A. Zewail; Somya Abdel-Rahman; Ahmed M. Naglah; Ahmed M. Shalaby. "Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods". EKB Journal Management System, 12, 2, 2013, 136-141. doi: 10.4103/1687-4315.124014
Zewail, M., Abdel-Rahman, S., Naglah, A., Shalaby, A. (2013). 'Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods', EKB Journal Management System, 12(2), pp. 136-141. doi: 10.4103/1687-4315.124014
Zewail, M., Abdel-Rahman, S., Naglah, A., Shalaby, A. Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods. EKB Journal Management System, 2013; 12(2): 136-141. doi: 10.4103/1687-4315.124014

Synthesis of the nonapeptide (B–B) of insulin B-chain using liquid and modified solid-phase methods

Egyptian Pharmaceutical Journal
Volume 12, Issue 2, July 2013, Page 136-141  XML PDF (598.7 K)
DOI: 10.4103/1687-4315.124014
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Authors
Mohamed A. Zewail; Somya Abdel-Rahman; Ahmed M. Naglah; Ahmed M. Shalaby
Abstract
Background and objective
This work embraced a systematic search for potentiated methodologies for peptide synthesis through a di-dimensional approach for convenient synthesis of the nonapeptide (B–B) of porcine insulin B-chain. Liquid-phase peptide synthesis (LPPS) and liquid–solid-phase peptide synthesis (modified SPPS) were used for the synthesis of this active part.
Materials and methods
A nonapeptide Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala-OH corresponding to B–B of porcine insulin B-chain was synthesized using different methods: LPPS and modified SPPS. Time consumption, yield and purity of all products using the different methods were compared with each other.
Results and conclusion
The results indicated that modified SPPS is advantageous, as it consumes less solvent per coupling and deprotection reaction, thereby reducing operating costs and solvent waste. Reducing side reactions result in racemization, cyclization or premature peptide formation. Modified SPPS produces peptides with yields and purity better than LPPS. Also, it can reduce the time of coupling and deprotection reactions.
Keywords
Amino acids; Insulin; Liquid phase; modified solid phase; Peptide Synthesis
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