Novel keratinase from marine NRC2aza exhibiting remarkable hide dehairing | ||||
| Egyptian Pharmaceutical Journal | ||||
| Volume 12, Issue 2, July 2013, Page 142-147 PDF (488.63 K) | ||||
| DOI: 10.4103/1687-4315.124016 | ||||
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| Author | ||||
| Azza M. Abdel-Fattah | ||||
| Abstract | ||||
| Background The isolation of the locally marine NRC2aza was characterized by the exceptional dehairing properties of its subtilisin-like keratinase. Objectives The aim of this work was to extract keratinase enzyme from the marine NRC2aza to be an alternative to sodium sulphide, which is the major pollutant from tanneries. Its unique nonactivity on collagen enhances its industrial potential. Material and methods Fermentation of the marine isolate NRC2aza on whole-feather medium was performed for keratrinase enzyme production. Extraction of the enzyme was carried out by solid-state fermentation (SSF). Results and conclusion NRC2aza have excellent characteristics of crude keratinase, producing 1680 U/ml in a shaking submerged culture (SmF) and 19 760 U/g using SSF after 4 days at pH 7. The effect of inoculum concentration on SSF was studied, whereby higher concentrations (150–200%) lowered the activity. Fractional precipitation of the enzyme by ammonium sulphate produced four fractions, of which 70% was the most active and produced remarkable hide dehairing. A new locally isolated spp. from marine ecosystem produced a highly active keratinase enzyme that exhibits remarkable hide dehairing. | ||||
| Keywords | ||||
| hide dehairing; keratin degradation; keratinase production; NRC2aza | ||||
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