Biochemical studies and biological activities on L-glutaminase from rhizosphere soil SAH2_CWMSG | ||||
| Egyptian Pharmaceutical Journal | ||||
| Volume 18, Issue 1, January 2019, Page 27-41 PDF (2.72 MB) | ||||
| DOI: 10.4103/epj.epj_32_18 | ||||
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| Authors | ||||
| Hassan M. Awad; Azza M.Noor El-Deen; El-Sayed E. Mostafa; Amany A. Hassabo | ||||
| Abstract | ||||
| Background and objective L-glutaminase (L-GLUNase) is a potential anticancer enzyme that hydrolyzes amide bond of L-glutamine to give glutamate and ammonium ion. It is used as an antioxidant, a flavor enhancing agent and a biosensor for glutamine level measurement. The aim was to produce L-GLUNase in high yield from a promising local isolate for many pharmaceutical applications. Materials and methods A total of 20 isolates for their capacity of L-GLUNase production were screened. A potent L-GLUNase producer, SAH2_CWMSG isolate, was identified by phenotypic and phylogenetic analysis. L-GLUNase was purified using ammonium sulfate followed by gel filtration on Sephadex G-100. The purified L-GLUNase was characterized, and its application as an antimicrobial, anticancer, and antioxidant agent was investigated. Results and conclusion The phylogenetic analysis of SAH2_CWMSG strain confirmed that the SAH2_CWMSG strain was most similar to (99%). It produced L-GLUNase activity of 58 U/ml under shake flask submerged fermentation. The purified L-GLUNase has the molecular weight of 55 kDa and K and V value of 1.314 mmol/l and 95.24 μ Me/min, respectively. Of the various physiochemical parameters tested, pH 7.5 and temperature 40°C were optimal for the enzyme activity. On the contrary, 10 mmol/l of Mn showed a slight increase in L-GLUNase activity. A promising sp. fully identified as SAH2_CWMSG (Gen Bank ID: KU720627) is an efficient source of L-GLUNase production. Therefore, it can be potentially used as enzyme supplement, which has many industrial and pharmaceutical applications. | ||||
| Keywords | ||||
| biological activities; L-glutaminse characterization; phenotypic and phylogenetic identification; rochei SAH2_CWMSG | ||||
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