adventitious root production of L. and antioxidants, total phenolic, and total flavonoids assessments | ||||
| Egyptian Pharmaceutical Journal | ||||
| Volume 18, Issue 3, July 2019, Page 216-227 PDF (1.28 MB) | ||||
| DOI: 10.4103/epj.epj_7_19 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mona M. Ibrahim ; Mohamed K. El-Bahr; Mohamed R. Rady
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| Abstract | ||||
| Background and objectives Chicory plant serves as a vegetable with higher nutritional value, having major antioxidant properties. The aim of this work was production of adventitious roots from subsp., L. and exploring the capacity of adventitious roots for antioxidant activities as well as determine their contents of total phenolic and flavonoids compounds compared with different parts of . Materials and methods For adventitious root induction, root segments were cultured on half-strength Murashige and Skoog solid medium supplemented with different concentrations of indole-3-butyric acid and 0.1 mg/l α-Naphthalene acetic acid. The cultures were incubated under total darkness and or 16/8 h (light/dark) photoperiod. Murashige and Skoog liquid medium with different carbon sources was used for adventitious root production. Two different solvents (aqueous ethanol and chloroform) were used for bioactive components extraction process. 2, 2′-diphenyl 1-Picryl-hydrazyl radical scavenging capacity (RSC) as well as total phenolic and flavonoides contents were estimated in produced adventitious roots compared with different plant parts (seeds, leaves, and roots). Results and conclusion Medium supplemented with 0.1 mg/l α-Naphthalene acetic acid and 1.0 mg/l indole-3-butyric acid recorded maximum adventitious root induction percentage (100%) in the dark condition. High-yield production of adventitious roots (6.60±0.5 g) was found in the liquid medium that contains sucrose as the carbon source. The aqueous ethanol extracts recorded higher RSC% values than chloroform extracts in all plant parts. Aqueous ethanol extract of seeds recorded maximum RSC% (92.8%) at 2.5 mg/ml of extract. Total phenolic contents showed maximum value with aqueous ethanol extract of seeds (18.17±0.40 mg/g of extract), whereas minimum value recorded with chloroform extract of seeds (0.28±0.05 mg/g of extract). The flavonoids contents showed maximum value also with aqueous ethanol extract of seeds (94.43±1.00 mg/g of extract), followed by aqueous ethanol extract of leaves (93.68±0.1 mg/g of extract), and minimum value with chloroform extract of leaves (2.60±0.18 mg/g of extract). | ||||
| Keywords | ||||
| Adventitious roots; Antioxidant activity; Phenolic and flavonoids | ||||
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