Ethanolic extract of sweet basil callus cultures as a source of antioxidant and sun-protective agents
Ibrahim, M., Danial, N., El-Bahr, M. (2023). Ethanolic extract of sweet basil callus cultures as a source of antioxidant and sun-protective agents. EKB Journal Management System, 22(1), 78-86. doi: 10.4103/epj.epj_124_22
Mona M. Ibrahim; Nancy Danial; Mohamed K. El-Bahr. "Ethanolic extract of sweet basil callus cultures as a source of antioxidant and sun-protective agents". EKB Journal Management System, 22, 1, 2023, 78-86. doi: 10.4103/epj.epj_124_22
Ibrahim, M., Danial, N., El-Bahr, M. (2023). 'Ethanolic extract of sweet basil callus cultures as a source of antioxidant and sun-protective agents', EKB Journal Management System, 22(1), pp. 78-86. doi: 10.4103/epj.epj_124_22
Ibrahim, M., Danial, N., El-Bahr, M. Ethanolic extract of sweet basil callus cultures as a source of antioxidant and sun-protective agents. EKB Journal Management System, 2023; 22(1): 78-86. doi: 10.4103/epj.epj_124_22

Ethanolic extract of sweet basil callus cultures as a source of antioxidant and sun-protective agents

Egyptian Pharmaceutical Journal
Volume 22, Issue 1, January 2023, Page 78-86  XML PDF (965.43 K)
DOI: 10.4103/epj.epj_124_22
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Authors
Mona M. Ibrahimorcid ; Nancy Danial; Mohamed K. El-Bahr
Abstract
Background
Basil ( L.) is a medicinal plant largely used in medicine, cosmetics, and cooking.
Objective
The current work aimed to improve the production of both phenolic and flavonoid compounds in the callus cultures of sweet basil ( L.), which can be used in cosmetics as antioxidant and sun-protection agents.
Materials and methods
Different combinations of growth regulators have been used to induce calli. Phenylalanine and salicylic acid have been used to enhance phenolics and flavonoids production. Quantitative analyses including total phenolics (TPC), total flavonoids, 2,2’-diphenyl 1-Picryl-hydrazyl radical scavenging activity, half-maximal inhibitory concentration (IC), correlation coefficient (R2) between antioxidant activity and both TPC and TFC, and sun-protective factor have been performed for both treatments and control.
Results and conclusions
Results reported that 1-naphthaleneacetic acid (NAA)+6-benzylaminopurine (BAP) was the best combination to induce calli tissue with good texture. The addition of 1.0 g/l phenylalanine for 2 weeks and 0.5 mm salicylic acid for 4 weeks were the best treatments to increase the production of phenolic and flavonoid components, and it showed the maximum % radical scavenging capacity. Higher correlation coefficient was found between % radical scavenging capacity and TPC compounds (0.83). The treatment of 1.0 g/l phenylalanine for 2 weeks indicated the lowest and best IC and it showed the maximum sun-protective factor value (36.50±0.003).
Keywords
Antioxidant activity; Ocimum basilicum L; phenolics and flavonoids; sun-protective factor
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