Production, characterization and application of crude fungal lipase from Aspergillus rubber OP520917 | ||||
| Egyptian Pharmaceutical Journal | ||||
| Volume 22, Issue 4, October 2023, Page 632-639 PDF (588.13 K) | ||||
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| Authors | ||||
| Rania Abdel Razik; Mona S. Shafei | ||||
| Abstract | ||||
| Background The pancreatic and stomach juices both include the naturally occurring lipase enzyme. Its job is to digest lipids and fats, which helps to keep the gallbladder working properly. Long chain triglycerides are hydrolyzed by microbial lipases, E.C 3.1.1.3 because they are capable of producing changed molecules, the lipase enzymes that come from microorganisms are theoretically versatile and useful for a wide range of industrial applications. Objective This study sought to determine the amount of crude lipase produced by A.rubber OP520917 during submerged fermentation and its use in removing oil from fabrics and breaking down chicken fats. Materials and methods Aspergillus rubber OP520917 was tested for lipase production using submerged fermentation; lipase activity was estimated, characterized and tested for industrial applications. Influence of: the pH; incubation times; surfactants and organic solvents and their concentration on the production of lipase by the selected strain were evaluated. Results and conclusion Aspergillus rubber OP520917 was identified strain for lipase production. The biochemical characterizations of lipolytic activity of A.rubber were studied and documented that the best temperature was 37°Cat pH 4 after 4 days of incubation time. The medium used for enzyme production contains olive oil as a carbon source, Moreover, 30% of hexane was the best organic solvent for the strain. Where the relative activity increased to 200%. The lipase activities were maximal (210,145 U/ml) in the presence of surfactants tween80 and tween20 respectively at 1% concentration. Furthermore, Lipase activity was also tested for removal of oil stains from the fabrics and the degradation of natural chicken fats with crude enzyme as industrial applications. The results showed that more than 74% of fats degraded after 5 days of the incubation period. | ||||
| Keywords | ||||
| Aspergillus rubber; destaining and degradation of animal fats application; lipase production | ||||
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