Study Of Methamphetamine Abuse Among Drug Abuse Positive Cases Attending Sohag Clinical Toxicology Laboratory By New Validated HPLC-DAD Method | ||||
| Ain Shams Journal of Forensic Medicine and Clinical Toxicology | ||||
| Volume 44, Issue 1, January 2025, Page 100-108 PDF (1.29 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajfm.2025.348496.1156 | ||||
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| Authors | ||||
Rania Ahmed Radwan  1; Nour Fadel Abouzied2; Ahmed Mora3; Ali M Hassan3; Mai Mostafa Abd ElKader4
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| 1Forensic medicine and clinical toxicology department, faculty of medicine, Sohag University, Egypt | ||||
| 2assistant professor of forensic medicine and clinical toxicology, faculty of medicine, sohag university, Egypt | ||||
| 3Chemistry Department, Faculty of Science, Al-Azhar University, Egypt | ||||
| 4Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Sohag University, Egypt | ||||
| Abstract | ||||
| Introduction: Substance abuse becomes a global challenge with alarming increase in use of crystal meth (shabu) in Egypt. For that, there is an urgent need to develop a reliable analytical method in routine operations. Aim: The current study aimed to develop and validate of a sensitive and accurate method for methamphetamine (meth) determination in human urine to evaluate prevalence of meth in Sohag population. Methodology: The study was applied on subjects attending Sohag Clinical Toxicology Laboratory for drug abuse testing, which exhibited primary positive results for amphetamine by rapid immunoassay dipstick (ABONtm). Amphetamine-positive cases were analyzed for meth by validated method on HPLC-DAD. Tert-butylmethyl ether (MTBE) in alkaline media was used to extract meth from urine and back-extracted into hydrochloric acid where propranolol was employed as the internal standard with extraction efficiency 77.77 %. Results: The calibration curve was linear (r2> 0.99) in the concentration range from0.25 to 3 µg /mL for methamphetamine. Limits of detection and quantification were 0.1 and 0.25 µg/mL, respectively. Intra- and inter-assay precisions for meth were within 2.75& 9.70 and 1.90 & 4.87%, respectively. Intra- and inter-assay accuracies were within (-6.67) &9.33 and (-2.78) & 6.67 %., respectively. The method revealed 51 cases of meth abuse in studied subjects. Their risk assessment and drug usage association were studied. Conclusion: The current method low limit of quantification (LOQ) of 0.25 µg/mL provides sensitive and adequate confirmatory physical evidence for the presence of meth in the urine. Recommendations: Routine meth screening is recommended viewing its increasing abuse and its hazardous health effects. | ||||
| Keywords | ||||
| Drug of abuse; methamphetamine; HPLC-DAD | ||||
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