Insights into Staphylococcus epidermidis in Farmed Nile Tilapia in Egypt: Molecular Characterization and Antibiotic Resistance | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 03 February 2025 PDF (871.24 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.328908.2435 | ||||
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| Authors | ||||
Abdelatty Saleh  1; Alaa Eldin Eissa2; Mohamed A Ghazy3; Sarah O.Makled4; Ahmed Abdel-Mawgood5
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| 1Biotechnology Program, School of Basic and Applied Sciences, Egypt-Japan University of Science and Technology, Alexandria, Egypt. | ||||
| 2Aquatic Animal Medicine and Management Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. | ||||
| 3Biotechnology Program, Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology (E-JUST), Alexandria, Egypt. | ||||
| 4Oceanography Department, Faculty of Science, Alexandria University, Alexandria, Egypt. | ||||
| 5Biotechnology Program, Basic and Applied Sciences Institute, Egypt-Japan University of Science and Technology (E-JUST), Alexandria, Egypt. | ||||
| Abstract | ||||
| Staphylococcus epidermidis is one of the causative agents of mass mortality in farmed Nile tilapia (Oreochromis niloticus) in Kafr Elsheikh Governorate, Egypt. Diseased farmed Nile tilapia were collected randomly during the fall of 2023 from farms suffering from mass mortalities. Clinical examination revealed severe lesions in the hepatopancreas and eye of the collected diseased fish. Based on morphological and biochemical analyses, the isolated bacteria were suggested to be Staphylococcus sp. Five of the nine isolates were selected for 16S rRNA sequencing based on their consistent phenotypic characterization criteria. One isolate was found to be Bacillus rugosus based on the 16S rRNA sequencing, which is non-pathogenic and out of the scope of this study. The four remaining isolates were found to be S. epidermidis, which have similarities to other S. epidermidis isolates. All isolated S. epidermidis were resistant to ampicillin, erythromycin, chloramphenicol, streptomycin, and amoxicillin; however, they were moderately susceptible to tetracycline and were sensitive to gentamicin and vancomycin. | ||||
| Keywords | ||||
| Nile tilapia; S. epidermidis; 16S rRNA; Phylogenetic analysis; Antibiogram | ||||
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