Innovative Diagnostic Model Based on Beta-Catenin, Tumor Necrosis Factor-Alpha, Interleukin 21 and CA19.9 Biomarkers for Follow-up of HCC Development After DAA Therapy for HCV Infection
Abdelsamea, S., El-Emshaty, H., Othman, O., Sultan, A., Ismail, H. (2025). Innovative Diagnostic Model Based on Beta-Catenin, Tumor Necrosis Factor-Alpha, Interleukin 21 and CA19.9 Biomarkers for Follow-up of HCC Development After DAA Therapy for HCV Infection. EKB Journal Management System, 68(5), 527-537. doi: 10.21608/ejchem.2025.339164.10880
Shimaa M. Abdelsamea; Hoda M. El-Emshaty; Othman ALI Othman; Ahmad M. Sultan; Hisham Ismail. "Innovative Diagnostic Model Based on Beta-Catenin, Tumor Necrosis Factor-Alpha, Interleukin 21 and CA19.9 Biomarkers for Follow-up of HCC Development After DAA Therapy for HCV Infection". EKB Journal Management System, 68, 5, 2025, 527-537. doi: 10.21608/ejchem.2025.339164.10880
Abdelsamea, S., El-Emshaty, H., Othman, O., Sultan, A., Ismail, H. (2025). 'Innovative Diagnostic Model Based on Beta-Catenin, Tumor Necrosis Factor-Alpha, Interleukin 21 and CA19.9 Biomarkers for Follow-up of HCC Development After DAA Therapy for HCV Infection', EKB Journal Management System, 68(5), pp. 527-537. doi: 10.21608/ejchem.2025.339164.10880
Abdelsamea, S., El-Emshaty, H., Othman, O., Sultan, A., Ismail, H. Innovative Diagnostic Model Based on Beta-Catenin, Tumor Necrosis Factor-Alpha, Interleukin 21 and CA19.9 Biomarkers for Follow-up of HCC Development After DAA Therapy for HCV Infection. EKB Journal Management System, 2025; 68(5): 527-537. doi: 10.21608/ejchem.2025.339164.10880

Innovative Diagnostic Model Based on Beta-Catenin, Tumor Necrosis Factor-Alpha, Interleukin 21 and CA19.9 Biomarkers for Follow-up of HCC Development After DAA Therapy for HCV Infection

Egyptian Journal of Chemistry
Volume 68, Issue 5, May 2025, Page 527-537  XML PDF (1.07 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2025.339164.10880
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Authors
Shimaa M. Abdelsamea1; Hoda M. El-Emshaty2; Othman ALI Othmanorcid 1; Ahmad M. Sultan3; Hisham Ismail email orcid 1
1Chemistry Department, Faculty of Science, Minia University, Minia, Egypt.
2Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
3Gastrointestinal Surgery Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abstract
Background & Aims: Our goals were to assess the predictive value of β-catenin, TNF-α, IL-21, and CA19-9 biomarkers and the diagnostic performance of a created model for early prediction of HCC after DAAs. Materials and Methods: ELISA was used to estimate serum levels of investigated biomarkers in 84 adult patients and 20 healthy individuals. To create an internally validated model predicts HCC development following DAA therapy of HCV patients, we employed linear regression analysis. Results: For HCV infected patients compared to the treated group, significant higher expression was detected in β-catenin (P < 0.0001) and TNF-α (P < 0.002) but a substantial increase was detected only in β-catenin (P < 0.0001) in LC. The AUC of β-catenin, TNF-α, IL-21, and CA19-9 for the potential diagnostic values of HCV-infected HCC patients from non-malignant individuals were 0.810, 0.829, 0.719, and 0.743; respectively. A newly developed MSB model based on β-catenin, TNF-α, IL-21, and CA19-9 had an AUC of 0.933 with sensitivity of 89.3%, and specificity of 93.7% at the best cut-off value of 3.09. Conclusion: The combination of β-Catenin, TNF-α, IL-21, and CA19-9 biomarkers constitute an outstanding diagnostic model for HCC (MSB model) that performs better pinpointing than each one alone.
Keywords
HCC; HCV; DAA; β-Catenin; Interleukin 21 (IL-21); Tumor Necrosis Factor-alpha (TNF-α); CA19-9
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