E. coli clinical isolates: Phylogrouping and virulence factors in Alexandria, Egypt | ||||
| Microbes and Infectious Diseases | ||||
| Volume 6, Issue 3, August 2025, Page 4035-4046 PDF (747.91 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.352346.2458 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Shahinda Rezk   1, 2; Ahmed Jamal1; Abeer Ghazal1; Aliaa Gamaleldin Aboulela 1
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| 1Department of Microbiology, Medical Research Institute, Alexandria University, Egypt. | ||||
| 2Institute of Virology, Medical Center-University of Freiburg, Freiburg 79104, Germany | ||||
| Abstract | ||||
| Background: E. coli is can be categorized into many pathotypes which represent groups of strains with specific pathogenic characteristics. This study aimed to identify phylogroups and different virulence factors in E coli isolated from clinical specimens. Methods: Fifty clinical E. coli isolates were included in this study. Identification and Antibiotic susceptibility testing were performed by Vitek® 2 compact system. Virulence genes detection and phylogroup gene alleles were performed using SYBR Green Real-time PCR. Results: The results revealed high antibiotic resistance; 62% of the isolates were MDR and 8% were XDR. The most prevalent virulence gene was papG (78%) while the least was hlyA (22%). Phylogroup B2 was the most predominant (52%), followed by (10%) group D, (8%) group C and group E each, (6%) group B1, (4%) clade1 and (2%) group F. Conclusion: Phylogroup B2 had all the virulence genes investigated in this study and it was more associated with sat and hlyA virulence genes. | ||||
| Keywords | ||||
| Phylogroups; Virulence genes; Antibiotic resistance | ||||
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