Identification of Hypervariable Domains in 12SrDNA for Enhanced Fish Species Barcoding from the Red Sea Regions of Egypt and Yemen | ||
| Sohag Journal of Sciences | ||
| Volume 10, Issue 1, March 2025, Pages 88-94 PDF (1.89 M) | ||
| Document Type: Regular Articles | ||
| DOI: 10.21608/sjsci.2024.312092.1219 | ||
| Authors | ||
| Asmaa Galal-Khallaf1; Aya Ibrahim Elberri1; Omir AbdelWahab Rabie1; Taha S. S. Baker2; Abdelaziz El-Sayed A. A. El-Sayed3, 4; Hamdy A. M. Soliman* 5; Khaled Mohammed-Geba1 | ||
| 1Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Egypt. | ||
| 2Department of Biology, College of Education, Ataq Shabowah University, Yemen. | ||
| 3Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt. | ||
| 4Department of Zoology, Faculty of Science, Islamic University of Madinah, Saudi Arabia. | ||
| 5Department of Zoology, Faculty of Science, Sohag University, Egypt. | ||
| Abstract | ||
| Ribosomal RNA (rRNA) genes, particularly 12SrDNA, have proven to be effective barcodes for fish species identification due to their secondary structure domains that exhibit interspecific variability. This study focused on identifying hypervariable domains within the 12SrDNA gene that contribute to its barcoding efficiency. Fish samples from Egypt and Yemen, representing various orders and families, were analyzed using 12SrDNA and COI gene sequencing. Four hypervariable domains (D2, D3, D5, D7) within 12SrDNA showed the highest divergence among species. Concatenating these domains significantly increased interspecific genetic distances. COI and 12SrDNA barcodes revealed 3 misidentified species (Plectorhinchus sordidus, Mulloidichthys flavolineatus, and Lethrinus nebulosus.), emphasizing the importance of focusing on specific hypervariable domains for accurate species identification. | ||
| Keywords | ||
| COI gene; DNA barcoding; Hypervariable domains, Fish species; 12SrDNA, Red Sea | ||
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