Study of the 16s rRNA gene sequences of Gut Microbiome in some Egyptian Patients with Colorectal Cancer

Fawzy, Moamen Ayman; Afifi, Mohamed T.; Ahmed, Shwikar M.; Shamseya, Ayman

doi:10.21608/svuijm.2023.220485.1609

	Study of the 16s rRNA gene sequences of Gut Microbiome in some Egyptian Patients with Colorectal Cancer
SVU-International Journal of Medical Sciences
Article 30, Volume 8, Issue 1, January 2025, Page 351-364 PDF (437.37 K)
Document Type: Original research articles
DOI: 10.21608/svuijm.2023.220485.1609
View on SCiNiTO
Authors
Moamen Ayman Fawzy ¹; Mohamed T. Afifi¹; Shwikar M. Ahmed²; Ayman Shamseya¹
¹Department of Internal Medicine ,Gastroenterology and Endoscopy Division , Faculty of Medicine, Alexandria University, Alexandria , Egypt.
²Department of Medical Microbiology and Immunology, Faculty of Medicine, Alexandria University, Alexandria , Egypt.
Abstract
Background: Colorectal cancer (CRC) is a heterogeneous disease of the gut epithelium up to 90% of disease risk is attributed to environmental factors like food and oncogenic bacteria. Objectives: The current study's objectives are to determine the gut microbiota that is probably linked to CRC and to evaluate the degree of variation between patients and healthy people. Patients and methods: The gut bacterial microbiome of 40 CRC patients and 40 healthy controls were analyzed using quantitative SYBR Green real-time PCR technique targeting 16S rRNA of selected bacteria. Results: The gut microbiome of the patients contained a higher relative abundance of Bacteroides as well as Clostridium difficile (C.difficile) and lower relative abundance of Firmicutes , Prevotella , Ruminococcus , Bifidobacteria and F. prausnitzii than controls. Shannon diversity index and Bray-Curtis dissimilarity index are statistically significant low in the CRC group compared to the control group. Conclusion: The current study showed evidence of changes in the gut Microbiome composition of CRC patients considering them less diverse and more dysbiotic compared to the healthy controls.
Keywords
Gut microbiome; Colorectal cancer; Dysbiosis; Real time PCR


Statistics Article View: 166 PDF Download: 87