Extraction, characterization of L-Asparaginase-Producing Fungi isolated from Diverse Soil Samples and exploring its antimicrobial activity: Emphasis on Aspergillus fumigatus for L-Asparagine Production
Mohamed, O., madkur, M., Elkhawaga, O., Gebreil, A. (2025). Extraction, characterization of L-Asparaginase-Producing Fungi isolated from Diverse Soil Samples and exploring its antimicrobial activity: Emphasis on Aspergillus fumigatus for L-Asparagine Production. EKB Journal Management System, 64(1), 1-7. doi: 10.21608/mjcc.2025.411313
Omnia Mohamed; Mai M. madkur; Omali Elkhawaga; Ahmed S. Gebreil. "Extraction, characterization of L-Asparaginase-Producing Fungi isolated from Diverse Soil Samples and exploring its antimicrobial activity: Emphasis on Aspergillus fumigatus for L-Asparagine Production". EKB Journal Management System, 64, 1, 2025, 1-7. doi: 10.21608/mjcc.2025.411313
Mohamed, O., madkur, M., Elkhawaga, O., Gebreil, A. (2025). 'Extraction, characterization of L-Asparaginase-Producing Fungi isolated from Diverse Soil Samples and exploring its antimicrobial activity: Emphasis on Aspergillus fumigatus for L-Asparagine Production', EKB Journal Management System, 64(1), pp. 1-7. doi: 10.21608/mjcc.2025.411313
Mohamed, O., madkur, M., Elkhawaga, O., Gebreil, A. Extraction, characterization of L-Asparaginase-Producing Fungi isolated from Diverse Soil Samples and exploring its antimicrobial activity: Emphasis on Aspergillus fumigatus for L-Asparagine Production. EKB Journal Management System, 2025; 64(1): 1-7. doi: 10.21608/mjcc.2025.411313

Extraction, characterization of L-Asparaginase-Producing Fungi isolated from Diverse Soil Samples and exploring its antimicrobial activity: Emphasis on Aspergillus fumigatus for L-Asparagine Production

Mansoura Journal of Chemistry
Article 1, Volume 64, Issue 1, January 2024, Page 1-7  XML PDF (1.18 MB)
Document Type: Original Article
DOI: 10.21608/mjcc.2025.411313
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Authors
Omnia Mohamed* 1; Mai M. madkur1; Omali Elkhawaga2; Ahmed S. Gebreil3
1Biochemistry division, Chemistry department, Faculty of Science, Mansoura University
2ChemistrBiochemistry division, Chemistry department, Faculty of Science, Mansoura Universityy
3Botany department, Faculty of Science, Mansoura University
Abstract
The objective of this study was to extract and characterize L-asparaginase from a thermophilic fungus, Aspergillus fumigatus which was isolated from different soil samples, and explore its antimicrobial activity. Six strains of fungi were isolated from soil samples at Mansoura University, identified, and screened for their ability to produce L-asparaginase using a modified Czapek-Dox medium with L-asparagine. Among the isolated fungi, Aspergillus fumigatus exhibited the highest L-asparaginase activity, identified through screening on Czapek's agar media. The organism was cultivated under submerged fermentation conditions, yielding L-asparaginase at its maximum activity at 40 °C and pH 8.0 after 30 minutes. The findings suggest that Aspergillus fumigatus from the soil samples has significant L-asparaginase activity and could be further explored for commercial L-asparagine production, given the enzyme's effective use as an antibacterial agent.
Keywords
Aspergillus fumigatus; L-Asparaginase, Optimization; Purification
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