Unveiling the First DNA Barcoding of Betta cf. uberis Fish (Anabantiformes: Osphronemidae) from Belitung Island, Indonesia | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 90, Volume 29, Issue 1, January and February 2025, Page 2023-2036 PDF (641.88 K) | ||||
| DOI: 10.21608/ejabf.2025.411476 | ||||
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| Author | ||||
| Syarif et al. | ||||
| Abstract | ||||
| Betta uberis, a member of the Betta coccina group, was first discovered on the Island of Borneo in 2006. A recent expedition to Belitung Island, in Dukong Village, a Swamp Forest Stream in East Belitung Regency, identified a species with strong morphological similarities to Betta uberis. To confirm the relationship between the species, a genetic analysis was conducted using DNA barcoding with the cytochrome oxidase subunit 1 (COI) gene. Molecular analysis revealed that the specimens from Belitung Island exhibit approximately 94.41% similarity to Betta uberis from Kalimantan. According to genetic guidelines, the specimens are considered non-identical if discrepancies exceed 3%, which is supported by the significant genetic divergence between them—a genetic distance of 0.057, corresponding to 33 DNA base variations out of 626. Phylogenetic analysis indicates that both the Belitung Island specimens and Betta uberis from Kalimantan belong to the same clade and share the closest genetic relationship among species in the Betta genus. Therefore, we have designated this species as Betta cf. uberis. Additionally, the DNA barcode for Betta cf. uberis has been recorded in the GenBank database under the accession identifier PQ675811.1. | ||||
| Keywords | ||||
| COI; Gene; DNA barcoding; Geographical distribution; New record | ||||
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