Evaluation of the Effect of Spirulina and Pioglitazone Individually and Combined in Ovalbumin-Induced Bronchial Asthma in Male Albino Rats | ||
| Suez Canal University Medical Journal | ||
| Article 2, Volume 26, Issue 12, December 2023, Pages 12-25 PDF (1.04 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scumj.2023.411872 | ||
| Authors | ||
| Heba M. M. Ahmed; Samah M. Elaidy* ; Eman Z. Abdelaziz; Hoda W. Elgawly | ||
| Department of Clinical Pharmacology, Faculty of Medicine, Suez Canal University, Egypt. | ||
| Abstract | ||
| Background: Peroxisome proliferator-activated receptor gamma (PPAR-γ) transcriptional pathway is crucial in regulating inflammation, including allergic subtypes. In experimental asthma, pioglitazone (PIO), a PPAR-γ agonist, exhibits protective and remodeling effects. Spirulina platensis extract (SPE), an extract from a type of blue-green algae, is a popular adjunctive therapy for allergic asthma. However, SPE's molecular targets in eosinophilic allergic asthma remain debatable. Aim: The current study looked at alterations in the PPAR-γ/nuclear factor (NF)-κβ pathway in ovalbumin (OVA)-induced allergic airway inflammation following treatment with SPE and PIO alone or together. Materials and Methods: A randomized controlled experimental study was held using fifty-six male Wistar rats. Sensitization and challenge by OVA were performed. SPE (500 mg/kg/day) and PIO (30 mg / kg / day) were given orally (from day 15 to day 28). Results: The severity of OVA-induced airway inflammation, as measured by elevated levels of total serum immunoglobulin (Ig)E, total and differential leukocytic counts in bronchoalveolar lavage (BAL) fluid, lung PPAR-γ gene expression, and NF-κβ expression in lung tissue, was significantly reduced by either SPE alone or in combination with PIO. Furthermore, the histological inflammatory and fibrotic lung scores decreased significantly following SPE and PIO, both individually and in combination. Of notice, the combination regimens displayed the highest improvements above individual SPE or, PIO. Conclusion: Spirulina's ability to stimulate PPAR-γ means it can mitigate the allergic airway inflammation caused by OVA. | ||
| Keywords | ||
| Allergy; Spirulina platensis; Peroxisome proliferator-activated receptor-gamma; Airway inflammation; pioglitazone; Nuclear factor-κβ | ||
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