Isolation, Identification, and A Statistical Optimization of Scleroglucan- Producing Athelia Rolfsii Did-2 Strain Isolated from Infected Phaseolus Vulgaris | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 68, Issue 10, October 2025, Page 699-710 PDF (1.78 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2025.330719.10685 | ||||
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| Authors | ||||
Ahamed E. El-Didamony1; Mohamed H. Kalaba1; Islam A. Elsehemy  2; Mohamed A.M. Farid 2; Saad A. Moghannem1
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| 1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt. | ||||
| 2Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, El- Buhouth St., Dokki, Giza, Egypt | ||||
| Abstract | ||||
| Athelia rolfsii Did-2 (OP600167) is a fungal strain that produces Scleroglucan, a non-ionic water-soluble biopolymer used in various industries. The study aimed to isolate and identify this fungal strain. Five fungal isolates from infected plants were tested, and strain Did-2 was selected as the highest producer (1.43 g/100 mL). The strain was identified using various international keys, including macroscopic, microscopic, and molecular studies. The partial sequence was deposited in GeneBank under the name Athelia rolfsii strain Did-2 18S ribosomal RNA gene with accession number OP600167. To maximize Scleroglucan yield, the Plackett-Burman design (PBD) and the central composite design response surface method (RSM) were employed. The optimized medium resulted in a 2-fold improvement (2.651 g/100 mL) in Scleroglucan yield | ||||
| Keywords | ||||
| Athelia rolfsii; scleroglucan; optimization Plackett-Burman; Response surface methodology | ||||
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