Purification and characterization of urease from leaves of Trigonella foenum-graecum
Ibrahim, A., Abdel-Aziz, A., El-Mougy, R., El-Shora, H. (2023). Purification and characterization of urease from leaves of Trigonella foenum-graecum. EKB Journal Management System, 62(4), 70-75. doi: 10.21608/mjcc.2023.412061
Amany A. Ibrahim; Abdel-Aziz F. Abdel-Aziz; Rehab A. El-Mougy; Hamed M. El-Shora. "Purification and characterization of urease from leaves of Trigonella foenum-graecum". EKB Journal Management System, 62, 4, 2023, 70-75. doi: 10.21608/mjcc.2023.412061
Ibrahim, A., Abdel-Aziz, A., El-Mougy, R., El-Shora, H. (2023). 'Purification and characterization of urease from leaves of Trigonella foenum-graecum', EKB Journal Management System, 62(4), pp. 70-75. doi: 10.21608/mjcc.2023.412061
Ibrahim, A., Abdel-Aziz, A., El-Mougy, R., El-Shora, H. Purification and characterization of urease from leaves of Trigonella foenum-graecum. EKB Journal Management System, 2023; 62(4): 70-75. doi: 10.21608/mjcc.2023.412061

Purification and characterization of urease from leaves of Trigonella foenum-graecum

Mansoura Journal of Chemistry
Volume 62, Issue 4, August 2023, Page 70-75  XML PDF (943.37 K)
Document Type: Original Article
DOI: 10.21608/mjcc.2023.412061
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Authors
Amany A. Ibrahim* 1; Abdel-Aziz F. Abdel-Aziz2; Rehab A. El-Mougy2; Hamed M. El-Shora3
11Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
2Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
3Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Abstract
Urease is amidohydrolase enzyme (EC: 3.5.1.5) splits urea into carbon dioxide and ammonia. The enzyme was purified from fenugreek (Trigonella foenum-graecum) leaves using ammonium sulphate precipitation (80%), phenyl sepharose, hydroxyapatite and sephadex G-200. The specific activity of the purified urease from sephadex G-200 was 210 U per mg protein with 6.6 % yield. The final fold of purification was 677. The optimal pH of the purified urease was 8.0 whereas the optimal temperature was 40°C. The purified urease exhibited appreciable thermal stability at 60 °C particularly in presence of bovine serum albumin (BSA). Also, the enzyme exhibited appreciable storage stability at 25°C.
Keywords
Urease; Trigonella foenum; graecum; Specific activity; Storage stability; Purification
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