Biochemical Evaluation between Urothelial bladder cancer and Upper Tract Urothelial Carcinomas
. Dawood, E., Awadalla, A., Shokeir, A., A. F, A. (2022). Biochemical Evaluation between Urothelial bladder cancer and Upper Tract Urothelial Carcinomas. EKB Journal Management System, 56(2), 8-12. doi: 10.21608/mjcc.2022.412098
Eman E. . Dawood; Amira Awadalla; Ahmed A Shokeir; Abdel-Aziz A. F. "Biochemical Evaluation between Urothelial bladder cancer and Upper Tract Urothelial Carcinomas". EKB Journal Management System, 56, 2, 2022, 8-12. doi: 10.21608/mjcc.2022.412098
. Dawood, E., Awadalla, A., Shokeir, A., A. F, A. (2022). 'Biochemical Evaluation between Urothelial bladder cancer and Upper Tract Urothelial Carcinomas', EKB Journal Management System, 56(2), pp. 8-12. doi: 10.21608/mjcc.2022.412098
. Dawood, E., Awadalla, A., Shokeir, A., A. F, A. Biochemical Evaluation between Urothelial bladder cancer and Upper Tract Urothelial Carcinomas. EKB Journal Management System, 2022; 56(2): 8-12. doi: 10.21608/mjcc.2022.412098

Biochemical Evaluation between Urothelial bladder cancer and Upper Tract Urothelial Carcinomas

Mansoura Journal of Chemistry
Volume 56, Issue 2, April 2022, Page 8-12  XML PDF (893.82 K)
Document Type: Original Article
DOI: 10.21608/mjcc.2022.412098
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Authors
Eman E. . Dawood* 1; Amira Awadalla2; Ahmed A Shokeir2; Abdel-Aziz A. F1
1Department of Chemistry, Biochemistry Division, Faculty of Science, Mansoura University,
2Center of Excellence for Genome and Cancer Research, Urology and Nephrology Center, Mansoura University, Mansoura
Abstract
Background: Urothelial carcinomas (UC) can be either in the upper or in the lower urinary tract or both.  Urothelial bladder cancer (UBC) is more common than upper tract urothelial carcinoma (UTUC). This research was designed to study the biochemical difference between UBC and UTUC through the comparison between Catalase and Nitric oxide and the measurements of CK20 and CD44 by flow cytometry. Methods: To study the discrepancy between UBC and UTUC. A prospective trial was carried out for 28 radical cystectomy and 18 nephrouretrectomy specimens of UBC and UTUC patients, respectively.  The activity of Catalase and concentration of Nitric oxide were assessed in in normal adjacent tissues, UTUC and UBC spectrophotometrically. CK20 and CD44 also were assessed in in normal adjacent tissues, UTUC and UBC by flow cytometry. Results: Comparison between UBC and UTUC regarding Catalase had significant difference (P value < 0.05) and comparison between UTUC and UBC regarding nitric oxide had significant difference (P value < 0.05), regarding CK20 in UTUC was positive (73.6%) and in UBC was positive CK20 (62.4%) and regarding CD44 in UTUC was positive (65.5%) and in UBC was negative CD44 (52.2%). Conclusions: Even though UTUC and UBC have the same origin, there is clear evidence that there is a biochemical difference between them. This biochemical difference could be the reason that UTUC is more aggressive than UBC
Keywords
Urothelial carcinoma; Catalase; Nitric oxide; CD44; CK20
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