Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress
Khaled, S., Abo Doma, A., Fahmy, E., Abdeltawab, F., Khalil, S., Abdelsattar, M. (2025). Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress. EKB Journal Management System, 65(2), 249-258. doi: 10.21608/ejbo.2024.312534.2965
Sandy Khaled; Ahmed H. Abo Doma; Eman M. Fahmy; Fatthy M. Abdeltawab; Shaimaa R.M. Khalil; Mohamed Abdelsattar. "Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress". EKB Journal Management System, 65, 2, 2025, 249-258. doi: 10.21608/ejbo.2024.312534.2965
Khaled, S., Abo Doma, A., Fahmy, E., Abdeltawab, F., Khalil, S., Abdelsattar, M. (2025). 'Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress', EKB Journal Management System, 65(2), pp. 249-258. doi: 10.21608/ejbo.2024.312534.2965
Khaled, S., Abo Doma, A., Fahmy, E., Abdeltawab, F., Khalil, S., Abdelsattar, M. Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress. EKB Journal Management System, 2025; 65(2): 249-258. doi: 10.21608/ejbo.2024.312534.2965

Alterations in the Expression of Senescence-Associated miRNAs and Their Target Genes in the mature flag leaf of wheat under Drought Stress

Egyptian Journal of Botany
Article 15, Volume 65, Issue 2, March 2025, Page 249-258  XML PDF (1.36 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2024.312534.2965
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Authors
Sandy Khaled1; Ahmed H. Abo Domaorcid 2; Eman M. Fahmy2; Fatthy M. Abdeltawab2; Shaimaa R.M. Khalil3; Mohamed Abdelsattar email orcid 4
1Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt. Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt
2Department of Genetics, Faculty of Agriculture, Ain Shams University, Shoubra Elkheima, Egypt
3Oil Crops Biotechnology Lab, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), 12619, Giza, Egypt
4Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt
Abstract
Targeted mRNAs cleavage is directed by a class of tiny RNAs known as microRNAs (miRNAs). Different plant processes are regulated by miRNAs, including senescence, responses to environmental stressors, and development. The current study identified the senescence regulation of miRNAs. It evaluated the impact of miRNA-directed modifications in expression of target genes of Triticum aestivum cultivars in response to drought. Two cultivars of spring wheat, Sahel-1and Gemiza-10 were used in this study. The Fv/Fm ratio, indicative of the maximum quantum efficiency of photosystem II, and the Performance Index (PI), an indicator for assessing sample vitality, exhibited significantly elevated values in the drought-tolerant cultivar relative to the drought-sensitive cultivar.Furthermore, quantitative PCR analyses for the miRNAs families; miR156, miR172, miR164, miR171, miR156, and miR319 and their putative target genes at reproductive phase under drought conditions in the two genotypes were performed. The up-regulation of AP2 in the Sahel-1genotype played a crucial role in leaf wilting and delaying aging under stress of drought. The miR164 down-regulation and up-regulation of NAC1 contributed to the delay of leaf wilting and senescence.
Keywords
microRNA; drought stress; Senescence; Triticum aestivum
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