Antioxidant and anticancer potential of successive extracts and column fractions of three microalgae were measured for optimum conditions | ||||
| Egyptian Journal of Botany | ||||
| Article 3, Volume 65, Issue 2, March 2025, Page 47-65 PDF (1.67 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2024.287638.2892 | ||||
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| Authors | ||||
Fatma El Zahraa Fadl  1; Sanaa Mahmoud Shanab 2; Emad Shalaby3
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| 1Botany &Microbiology department,Faculty of Science,Cairo university | ||||
| 2Botany and Microbiology department, Faculty of Science, Cairo University, Egypt | ||||
| 3Biochemistry, Agriculture, Cairo University | ||||
| Abstract | ||||
| Three green microalgal species ( Chlamydomonas reinhardtii , Dictyochloropsis splendida , Muriella sp.) were cultured under four stress culture conditions (nitrogen concentrations , light intensity , photoperiod , salinity). The aim of the present study is to evaluate the algal growth parameters , antioxidant and anticancer efficiencies of the stressed biomasses. Extraction was performed by hexane , ethyl acetate , methanol and produced 36 extracts (E1-E36 ). Antioxidant activity of the 36 crude extracts were performed by radical DPPH & non-radical KMnO4. E14 of D. splendida cultivated on 3g L nitrogen was the promising crude extract exhibited the greatest antioxidant activity by both methods. Fractioation of E14 was done by silica gel column chromatography produced 11 fractions (F1—F11) . Greater antioxidant activity of F5, F8 occurred compared to antioxidant standards (Ascorbic acid, BHT). High Performance Liquid Chromatography ( HPLC ) analyses of E14 , F5 , F8 were evaluated and 4 ,5 , 6 Phenolic compounds were produced respectively. Anticancer activity of E14, F5 , F8 as well as the mixture of F5+ F8 was determined against three cancer cell lines ( HepG2 , MCF7 & Pc3 ) against MTT assay. The obtained results showed that fractions and mixture of fractions exhibited greater activity and lower IC50 compared to their crude extract E14 . | ||||
| Keywords | ||||
| Microalgae; Chlamydomonas; Muriella; Dictyochloropsis; antioxidant; anticancer | ||||
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