In-vitro investigation of the anti-diabetic and anti-cancer potential of Acacia pennata leaves extract and its phenolic content | ||||
| Egyptian Journal of Chemistry | ||||
| Articles in Press, Accepted Manuscript, Available Online from 25 February 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2025.335031.10771 | ||||
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| Authors | ||||
Inas Z.A. Abdallah1; Hagar Farid Elbakry 2; Sahar Y. Al-Okbi  2; Mona S.M. Abd El-kader1
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| 1Department of Nutrition and Food Science, Faculty of Home Economics, Helwan University, Egypt | ||||
| 2Department of Nutrition and Food Sciences, National Research Centre, Cairo, Egypt. | ||||
| Abstract | ||||
| Diabetes is a significant public health problem in Egypt and worldwide. In addition hepatocellular carcinoma is the sixth common malignancy in the world. Therefore, the current study was conducted to evaluate the in-vitro efficiency of Acacia pennata leaves aqueous ethanol extract (APLE) in mitigating hyperglycemia and limiting the growth of HepG2 cancer cell lines. Methodology involved HPLC analysis of APLE for determination of phenolic compounds. Alpha glucosidase and α-amylase inhibitory activities of APLE were estimated. The IC50 of APLE against hepatocellular carcinoma cell lines was assessed. Acute toxicity test of the extract was done. Results showed that APLE was rich in phenolic compounds of which quercetin (343 μg/g) was the major identified flavonoid. APLE showed alpha amylase inhibitory activity with IC50 of 1.05 mg/ml and alpha glucosidase inhibitory activity with IC50 of 0.34 mg/ml. APLE showed anti-cancer effect against HepG2 cell lines with IC50 of 114 μg/ml. Safety of the APLE was confirmed in senile mice with no death or signs of acute toxicity appeared on mice after 24 hours of oral administration of up to 10 g APLE/kg mice body weight. Conclusion: Acacia pennata leaves aq. alcohol extract has in-vitro anti-diabetic activity as well as anti-cancer effect against HepG2 cell lines which could be ascribed to the presence of phenolic compounds. | ||||
| Keywords | ||||
| Acacia pennata; alpha-glucosidase; alpha-amylase; HepG2 cell line; phenolic compounds | ||||
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