Phenotypic versus molecular techniques for the detection of carbapenemases among Egyptian carbapenem-resistant A. baumannii isolates. | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 25 February 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.349151.2424 | ||||
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| Authors | ||||
Kholoud Baraka   ; Rania Abozahra ; Fatma Ali; Sarah Abdelhamid
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| Microbiology and Immunology Department, Faculty of Pharmacy, Damanhour University, El Behira, Egypt | ||||
| Abstract | ||||
| Background: Carbapenem resistance in A. baumannii is primarily driven by the production of 4 classes of β-lactamase enzymes. Aim: The aim of this study was to compare between phenotypic and molecular techniques for the detection of carbapenemases among CR-AB isolates. Methods: Thirty seven A. baumannii isolates were collected and carbapenems susceptibility testing was performed by disc diffusion and agar dilution methods. Carbapenemases were detected phenotypically by MHT, CIM, CDT, CarbAtcineto NP test, and boronic acid disc test. Carbapenemases and inertion sequences genes were detected by PCR. Results: For MHT, CIM and CDT, 62.2%, 54.1%, and isolates 78.4% were positive, respectively. For Boronic acid test, 56.8% isolates were positive with imipenem and 67.6% isolates with meropenem. CarbAcineto NP test were positive in 94.5% isolates. blaOXA-58 gene wasn't detected in any of our isolates. blaKPC gene was detected in 21.6% isolates. blaVIM and blaNDM genes were detected in 78.4% and 92.9% isolates, respectively. blaOXA-51 and blaOXA-23 genes were detected in 100% and 86.5% isolates, respectively; however, blaOXA-24 gene was detected in 2.7% isolate. Regarding insertion sequences, ISAbal and ISAba3 genes were detected in 100% isolates, while ISAba2 gene was detected in only 18.9% isolates. Conclusion: CarbAcineto NP test demonstrated superior sensitivity which was closely aligned with PCR results. Combination of phenotypic and molecular methods provides a comprehensive approach to carbapenemase detection in clinical isolates of A. baumannii. | ||||
| Keywords | ||||
| Acinetobacter; carbapenemase; insertion sequence; CIM; CDT | ||||
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