Molecular Evaluation of the Therapeutic Potential of DNA-Based Vaccines Against Experimentally Induced Lung Cancer
Hassab El-Nabi, S., Zidan, A., El. Ghayesh, N., Abdel Aziz, K., Mokhamer, E. (2025). Molecular Evaluation of the Therapeutic Potential of DNA-Based Vaccines Against Experimentally Induced Lung Cancer. EKB Journal Management System, 11(1), 20-36. doi: 10.21608/jbaar.2025.414372
S. E. Hassab El-Nabi; Abdel Aziz A. Zidan; Nourhan K. El. Ghayesh; Karolin K. Abdel Aziz; EL Hassan M. Mokhamer. "Molecular Evaluation of the Therapeutic Potential of DNA-Based Vaccines Against Experimentally Induced Lung Cancer". EKB Journal Management System, 11, 1, 2025, 20-36. doi: 10.21608/jbaar.2025.414372
Hassab El-Nabi, S., Zidan, A., El. Ghayesh, N., Abdel Aziz, K., Mokhamer, E. (2025). 'Molecular Evaluation of the Therapeutic Potential of DNA-Based Vaccines Against Experimentally Induced Lung Cancer', EKB Journal Management System, 11(1), pp. 20-36. doi: 10.21608/jbaar.2025.414372
Hassab El-Nabi, S., Zidan, A., El. Ghayesh, N., Abdel Aziz, K., Mokhamer, E. Molecular Evaluation of the Therapeutic Potential of DNA-Based Vaccines Against Experimentally Induced Lung Cancer. EKB Journal Management System, 2025; 11(1): 20-36. doi: 10.21608/jbaar.2025.414372

Molecular Evaluation of the Therapeutic Potential of DNA-Based Vaccines Against Experimentally Induced Lung Cancer

Journal of Bioscience and Applied Research
Volume 11, Issue 1, March 2025, Page 20-36  XML PDF (1.52 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2025.414372
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Authors
S. E. Hassab El-Nabi1; Abdel Aziz A. Zidan2; Nourhan K. El. Ghayesh2; Karolin K. Abdel Aziz2; EL Hassan M. Mokhamer* 2
1Zoology Department, Faculty of Science, Menoufia University, Egypt.
2Zoology Department, Faculty of Science, Damanhur University, Egypt
Abstract
Lung cancer, or lung carcinoma, is a malignant lung tumor characterized by uncontrolled cell growth in lung tissue. In Egypt, it has high morbidity and mortality rates.  Polyinosinic-polycytidylic acid (poly (I: C)) is a double-stranded RNA analog, a potent immunostimulatory agent that activates multiple components of the immune system, and cyclophosphamide is an alkylating cytotoxic drug that inhibits DNA synthesis to kill tumor cells. Transforming Growth Factor Beta 1 (TGF-β1) plasmid vaccine aims to neutralize or downregulate the immunosuppressive effects of TGF-β1, enhancing immune system activity against cancer cells. This study investigated the impact of the TGF-β1 plasmid vaccine, alone or in combination with cyclophosphamide and poly (I: C), on lung metastasis of B16/F10 murine melanoma cells in syngeneic C57BL/6 mice. The results demonstrated overexpression of epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) proteins in tumor-bearing or cyclophosphamide-treated mice. In contrast, the vaccine ameliorated the overexpression of EGFR and VEGF.
These findings suggest that the TGF-β1 plasmid vaccine, especially in combination with poly (I: C), effectively mitigates lung cancer progression in mice by inhibiting tumor growth, enhancing cell survival, and reducing the chemotherapy side effects. This study highlights the potential of the TGF-β1 plasmid vaccine as a promising therapeutic strategy for lung cancer.
Keywords
Lung cancer; B16/F10 melanoma cells; Poly(I:C); cyclophosphamide; TGF-β1 plasmid vaccine; EGFR
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