Natural polyphenols target the TGF-β/caspase-3 signaling pathway in CCl4-induced liver fibrosis in rats

Abu-Baih, Rania H.; Bayoumi, Asmaa M. A.; Ibrahim, Ahmed R. N.; Ewees, Mohamed G.; Abdelraheim, Salama R.

doi:10.21608/jabps.2019.13319.1049

	Natural polyphenols target the TGF-β/caspase-3 signaling pathway in CCl4-induced liver fibrosis in rats
Journal of advanced Biomedical and Pharmaceutical Sciences
Article 1, Volume 2, Issue 4, October 2019, Page 129-134 PDF (994.28 K)
Document Type: Original Article
DOI: 10.21608/jabps.2019.13319.1049
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Authors
Rania H. Abu-Baih ¹; Asmaa M. A. Bayoumi ²; Ahmed R. N. Ibrahim ²; Mohamed G. Ewees ³; Salama R. Abdelraheim ⁴
¹Minia University Hospital, 61519 Minia, Egypt
²Department of Biochemistry, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
³Department of Pharmacology and Toxicology, Faculty of Pharmacy, Nahda University, 62513 New Beni Suef, Egypt
⁴Department of Biochemistry, Faculty of Medicine, Minia University, 61519 Minia, Egypt
Abstract
Background/Aims: Liver fibrosis presents a worldwide problem. There is an increasing interest in the studying of natural compounds with free radicals scavenging capacity to protect against liver fibrosis. This study investigated the actions of resveratrol and curcumin as two natural polyphenols against CCl4-induced liver fibrosis. Materials and Methods: Animals were divided into four groups: Normal control group; CCl4 only group; Res group (resveratrol + CCl4); Cur group (curcumin + CCl4). Serum ALT and AST were evaluated. Assessment of liver MDA, GSH and catalase was performed. Caspase-3 expression was evaluated by western blotting. Levels of TGF-β1 and TGF-β2 mRNA were determined using qRT-PCR. In addition, liver histopathological sections were examined. Results: We found that serum ALT and AST were elevated by CCl4, however, they were significantly reduced by resveratrol and curcumin. Furthermore, CCl4 induced oxidative stress, stimulated fibrosis and apoptosis. Resveratrol and curcumin significantly attenuated the state of oxidative stress, fibrosis and apoptosis. Conclusion: We conclude that resveratrol and curcumin are natural polyphenols that can protect against liver fibrosis not only via antioxidant, but also via anti-fibrotic and anti-apoptotic potentials.
Keywords
Liver fibrosis; Resveratrol; Curcumin; TFG-β; Caspase-3


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