The expression pattern of Bmi 1 can predict the therapeutic potential of piperine in experimentally induced hamster buccal pouch carcinoma (Histological and Immunohistochemical Studies)
Mohamed, H., ibrahim, A., Elshafei, A., ahmed, M. (2025). The expression pattern of Bmi 1 can predict the therapeutic potential of piperine in experimentally induced hamster buccal pouch carcinoma (Histological and Immunohistochemical Studies). EKB Journal Management System, 28(1), 61-70. doi: 10.21608/ajdsm.2023.221923.1439
Hassan Ali Mohamed; amr mohamed ibrahim; Ahmed Mohamed Elshafei; mohamed mahmoud ahmed. "The expression pattern of Bmi 1 can predict the therapeutic potential of piperine in experimentally induced hamster buccal pouch carcinoma (Histological and Immunohistochemical Studies)". EKB Journal Management System, 28, 1, 2025, 61-70. doi: 10.21608/ajdsm.2023.221923.1439
Mohamed, H., ibrahim, A., Elshafei, A., ahmed, M. (2025). 'The expression pattern of Bmi 1 can predict the therapeutic potential of piperine in experimentally induced hamster buccal pouch carcinoma (Histological and Immunohistochemical Studies)', EKB Journal Management System, 28(1), pp. 61-70. doi: 10.21608/ajdsm.2023.221923.1439
Mohamed, H., ibrahim, A., Elshafei, A., ahmed, M. The expression pattern of Bmi 1 can predict the therapeutic potential of piperine in experimentally induced hamster buccal pouch carcinoma (Histological and Immunohistochemical Studies). EKB Journal Management System, 2025; 28(1): 61-70. doi: 10.21608/ajdsm.2023.221923.1439

The expression pattern of Bmi 1 can predict the therapeutic potential of piperine in experimentally induced hamster buccal pouch carcinoma (Histological and Immunohistochemical Studies)

Al-Azhar Journal of Dental Science
Volume 28, Issue 1, February 2025, Page 61-70  XML PDF (1.9 MB)
Document Type: Original Article
DOI: 10.21608/ajdsm.2023.221923.1439
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Authors
Hassan Ali Mohamed email 1; amr mohamed ibrahim1; Ahmed Mohamed Elshafei2; mohamed mahmoud ahmed3
1Oral and Dental Pathology Department, Faculty of Dental Medicine (Boys- Cairo), Al-Azhar University, Egypt.
2Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, (Boys-Cairo), Al-Azhar University, Egypt.
3Oral and Dental Pathology Department, Faculty of Dental Medicine (Boys-Cairo), Al-Azhar University, Egypt.
Abstract
Abstract: One of the goals of the study was to investigate the expression pattern of Bmi1 of treated hamsters with piperine after experimentally induced HBP carcinoma. Material and methods: Thirty male Syrian hamsters were randomly split into three groups (G(s)) of ten. Hamsters in groups II and III had 7, 12-dimethylbenz (a) anthracene (DMBA) painted into their right hamster buccal pouch (HBP) three times a week for 14 weeks, whereas hamsters in group I (control) were left untreated for 22 weeks. After that, the animals in GII (positive control) were left with no additional treatment for other 8 ws (total period 22 ws), whereas those in GIII were treated by piperine (50 mg/kg, orally) daily for 4ws, and then, animals were left with no additional treatment for other 4 ws (total period 22 ws). After termination of the experiment, After euthanizing the animals, HBP were surgically removed, cleaned, fixed, and processed for a histological analysis and categorization using H&E stain, and immunohistochemical (IHC) staining utilizing Bmi1 cancer stem cell(CSC) antibody. Results: The gap between GII and GIII was statistically very significant (p value < 0.001) regarding to tumor volume, depth of invasion( DOI) and regarding nuclear expression of Bmi1which revealed that, GI had the smallest average surface area (9.71%), whereas GII had the largest average surface area (65.88%), followed by GIII Conclusions: piperine could inhibit HBP squamous cell carcinoma (SCC) in addition to, downregulation of Bmi1 can predict the therapeutic potential of piperine in HBP SCC.
Keywords
HBP carcinoma; piperine; Bmi1
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