Biotechnological Potential of a Thermostable and pH-Stable Protease from Bacillus sp. AM12: An Innovative Approach for Wool Biodegradation and Industrial Applications
Hussein, A., Kabeil, S., El-Tokhy, N., Omar, S., Elbahloul, Y. (2025). Biotechnological Potential of a Thermostable and pH-Stable Protease from Bacillus sp. AM12: An Innovative Approach for Wool Biodegradation and Industrial Applications. EKB Journal Management System, 1(4), 17-29. doi: 10.21608/fpmpeb.2025.414829
Ahmed Hussein; Sanaa Kabeil; Nabil El-Tokhy; Sanaa Omar; Yasser Elbahloul. "Biotechnological Potential of a Thermostable and pH-Stable Protease from Bacillus sp. AM12: An Innovative Approach for Wool Biodegradation and Industrial Applications". EKB Journal Management System, 1, 4, 2025, 17-29. doi: 10.21608/fpmpeb.2025.414829
Hussein, A., Kabeil, S., El-Tokhy, N., Omar, S., Elbahloul, Y. (2025). 'Biotechnological Potential of a Thermostable and pH-Stable Protease from Bacillus sp. AM12: An Innovative Approach for Wool Biodegradation and Industrial Applications', EKB Journal Management System, 1(4), pp. 17-29. doi: 10.21608/fpmpeb.2025.414829
Hussein, A., Kabeil, S., El-Tokhy, N., Omar, S., Elbahloul, Y. Biotechnological Potential of a Thermostable and pH-Stable Protease from Bacillus sp. AM12: An Innovative Approach for Wool Biodegradation and Industrial Applications. EKB Journal Management System, 2025; 1(4): 17-29. doi: 10.21608/fpmpeb.2025.414829

Biotechnological Potential of a Thermostable and pH-Stable Protease from Bacillus sp. AM12: An Innovative Approach for Wool Biodegradation and Industrial Applications

Future Perspectives of Medical, Pharmaceutical and Environmental Biotechnology
Volume 1, Issue 4, February 2025, Page 17-29  XML PDF (778.91 K)
Document Type: Research Article (Original Research)
DOI: 10.21608/fpmpeb.2025.414829
View on SCiNiTO View on SCiNiTO
Authors
Ahmed Hussein1; Sanaa Kabeil email 2; Nabil El-Tokhy3; Sanaa Omar1; Yasser Elbahloul1
1Botany and Microbiology Department, Faculty of Science, Cairo University
2Proteins Research Department, Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications New Borg El-Arab City, Alexandria, Egypt
33Pharmaceutical Bio-Products Research Department, Institute of Genetic Engineering and Biotechnology, City of Scientific Research and Technological Applications New Borg El-Arab City, Alexandria, Egypt
Abstract
Biodegradation of wool fibers using microbial proteases is an environmentally friendly sustainable biotechnology used as a substitute for regular chemical treatments. This study isolated and identified a potent wool-degrading Bacillus sp. AM12 from soil and water samples in Egypt. The isolated bacterium's maximum strong proteolytic activity (180.7 U/ml) was detected upon growth on the APPB medium, confirming wool as an inducer. The crude enzyme was identified as an alkaline protease enzyme with optimal activity at pH 10 and 60°C. Wool degradation by crude enzyme was confirmed by a decrease of weight without shrinkage, while SEM imaging revealed surface smoothing and modifications. Anion exchange and gel filtration chromatography were used for enzyme purification, resulting in a 2.66-fold increase in purity and a specific activity of 5.6 U/mg. SDS-PAGE analysis of the purified enzyme predicted its molecular weight at 24 kDa. Moreover, the enzyme retained its thermostability, pH stability and over 80% activity in commercial detergents such as SDS and Tween-80, confirming its industrial biotechnological compatibility.  These findings showed the potential of Bacillus sp. AM12 protease as a sustainable and effective alternative in industrial wool treatment.
Keywords
Protease; Thermostable; Industrial Biotechnology
Statistics
Article View: 165
PDF Download: 90