Study of entB Gene and Some Quinolone Resistance Genes (qnrB and acc(6’)-Ib-cr) of Klebsiella pneumoniae in Community-Acquired Infections in Kirkuk City | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 34, Issue 3, July 2025, Pages 173-182 PDF (834.83 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.357953.1463 | ||
| Authors | ||
| Bahasht Y. Ezzulddin* ; Mohammed Y. Nooraldeen | ||
| Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Kirkuk, Northern Technical University, Iraq Center of Technical Research, Northern Technical University, Iraq | ||
| Abstract | ||
| Background: Community-acquired Klebsiella pneumoniae (CAKP) infections arise in people outside hospital environments and can affect various body parts. There is a growing problem with antibiotic resistance, particularly fluoroquinolone resistance, which limits treatment choices for CAKP infections. Objective: This study aimed to detect the entB gene and some fluoroquinolone resistance genes qnrB and aac(6′)-Ib-cr of K. pneumoniae in Community-acquired infections. Methodology: Two hundred fifty-six different clinical samples were collected from outpatients of both sexes suffering from community-acquired infections aged less than one to eighty years. K. pneumoniae was isolated and identified using traditional microbiological methods, and subsequently confirmed with the VITEK 2 compact system. Antibiotic sensitivity was assessed for Gemifloxacin and Norfloxacin by disc diffusion method, and the minimum inhibitory concentration of Ciprofloxacin was evaluated using VITEK 2 AST-GN-419 cards. EntB, qnrB, and aac(6’)-Ib-cr genes were detected using polymerase chain reaction (PCR). Results: Only thirty-eight non-duplicate K. pneumoniae isolates were identified. The antibiotic susceptibility test revealed 55.3% resistance of isolates to the ciprofloxacin, 50% to the gemifloxacin, and 42.1% to norfloxacin. PCR analysis showed that of all 38 K. pneumoniae isolates, 100% harbored the entB gene. Among 38 isolates, only 26 isolates were phenotypically resistant to fluoroquinolone and they were involved in the molecular detection of plasmid mediated-quinolone-resistant genes (PMQR), the acc(6')-Ib-cr gene was identified in 13 (50%) isolates. In contrast, qnrB was found in 9 (34.6%). Conclusion: A high prevalence of virulence factors can enhance the pathogenicity of K. pneumoniae in infections acquired in the community. Among the factors determining PMQR, the aac (6')-Ib-cr gene tends to be prevalent, followed by the qnrB gene. | ||
| Keywords | ||
| Community-acquired Klebsiella pneumoniae; entB; qnrB; aac (6')-Ib-cr; PCR | ||
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