Impact of Obeticholic Acid and Moringaoleifera Leaf Extract on Arsenic induced Hepatotoxicity and Related Immunohistochemistry Changes in Adult Male Albino Rats | ||
| International Journal of Medical Arts | ||
| Article 3, Volume 7, Issue 5, May 2025, Pages 5658-5666 PDF (2.05 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ijma.2025.356224.2118 | ||
| Authors | ||
| Mohamed Ali Mahmoud Abbas* 1; Fatma Noaman1; Hamed Osman2; Ashraf Emara2 | ||
| 1Department of Medical Physiology, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt. | ||
| 2Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt. | ||
| Abstract | ||
| Background: A naturally occurring poison, arsenic can be found in food, soil, and water. Drinking water tainted with arsenic is the main way of exposure. Obeticholic acid [OCA] has been studied in a variety of conditions, its ability to treat hepatic damage is yet unknown. Aim of the work: This study aimed to discover how the administration of obeticholic acid and Moringa oleifera leaf extract affects the hepatotoxicity caused by arsenic in adult male albino rats. Methods: Fifty adult male albino rats of the local strain were selected. Rats were divided into 5 equal groups: Group I [control], Group II [Hepatotoxicity], Group III [Hepatotoxicity Plus obeticholic acid], Group IV [Hepatotoxicity Plus Moringaoleifera leaf extract] and Group V [Hepatotoxicity Plus obeticholic acid and Moringaoleifera leaf extract]. Rats were slaughtered at the end of the experiment, and blood samples were obtained to measure high density lipoprotein [HDL], low density lipoprotein [LDL], triglycerides [TG], total cholesterol, aspartate transaminase [AST], alanine transaminase [ALT], alkaline phosphatase [ALP], and L-lactate dehydrogenase [LDH]. Superoxide dismutase [SOD], glutathione [GSH], albumin, malondialdehyde [MDA], catalase [CAT], and tumour necrosis factor alpha [TNF α]. Nuclear factor kappa [NF-kB] immunohistochemistry and liver histopathology analysis validated the findings. Results: Arsenic can cause significant increase in Cholesterol, TGs, LDL, AST, ALT, ALP, MDA, SOD, GSH, TNF α associated with significant decrease in HDL, LD, Albumin, CAT. The administration of Obeticholic acid or Moringaoleifera leave extract resulted in significant improvement in the form of significant reduction of Cholesterol, TGs, LDL, AST, ALT, MDA, SOD, GSH, TNF α associated with significant increase in HDL, Albumin, CAT. Obeticholic acid or Moringaoleifera leaf extract showed comparable improvement in liver functions but their combined treatment showed better improvement. Conclusion: Obeticholic acid and Moringaoleifera leave extract can equally prevent hepatotoxicity in arsenic-induced hepatotoxicity, but the co-administration showed superior outcome. | ||
| Keywords | ||
| Obeticholic Acid; Moringaoleifera; Arsenic; Oxidative stress; Hepatotoxicity | ||
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