Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines
Tamam, F., ElHefnawi, M., Elsayed, G., El-Beih, N., El-Hussieny, E., Yahya, S. (2025). Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines. EKB Journal Management System, 17(1), 115-129. doi: 10.21608/eajbsz.2025.416999
Fatma Tamam; Mahmoud ElHefnawi; Ghada H. Elsayed; Nadia M. El-Beih; Enas A. El-Hussieny; Shaymaa M.M. Yahya. "Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines". EKB Journal Management System, 17, 1, 2025, 115-129. doi: 10.21608/eajbsz.2025.416999
Tamam, F., ElHefnawi, M., Elsayed, G., El-Beih, N., El-Hussieny, E., Yahya, S. (2025). 'Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines', EKB Journal Management System, 17(1), pp. 115-129. doi: 10.21608/eajbsz.2025.416999
Tamam, F., ElHefnawi, M., Elsayed, G., El-Beih, N., El-Hussieny, E., Yahya, S. Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines. EKB Journal Management System, 2025; 17(1): 115-129. doi: 10.21608/eajbsz.2025.416999

Elucidation of Hsa-miR-98's Role in Oncogenicity and Oxidative Stress in Breast Cancer Cell Lines

Egyptian Academic Journal of Biological Sciences, B. Zoology
Volume 17, Issue 1, June 2025, Page 115-129  XML PDF (936.85 K)
Document Type: Original Article
DOI: 10.21608/eajbsz.2025.416999
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Authors
Fatma Tamam1; Mahmoud ElHefnawi2; Ghada H. Elsayed3; Nadia M. El-Beih1; Enas A. El-Hussieny1; Shaymaa M.M. Yahya3
1Department of zoology faculty of science Ain shams university,Cairo.Egypt.
2Biomedical informatics and chemo informatics group, informatics and systems department, National Research Center, Dokki, Giza, Egypt.
3Hormones Department, Medical Research and Clinical Studies Institute, and Stem Cell lab, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, Giza, Egypt.
Abstract
The role of miR -98 is controversial in the literature which we aimed to clarify in breast cancer cell lines.  To elucidate the role of miR-98, mimic and inhibitor were transfected into MCF-7and MDA-MB-231 cell lines. Q-RT-PCR was used to analyze Wnt pathway gene expression. Function analysis including wound healing, and determining oxidative stress parameters were conducted. miR-98 was up-regulated in most breast cancer tissue databases. Bioinformatics analysis target prediction and enrichment analysis clarified that miR-98 is incorporated in oxidative stress pathway. It targets several genes from which HIF-1A and VEGFA which were selected for real time analysis. Q-RT- PCR results revealed that HIF-1A and VEGFA were non-significantly increased upon inhibition of miR-98 in MCF-7 while HIF-1A and VEGFA were significantly decreased upon inhibition of miR-98 in MDA-MB-231. On the other hand, the level of catalase which is ROS tracker enzyme in cell lysate of MCF-7 was significantly increased upon inhibition of miR-98. miR-98 triggered the cells of MCF-7 and MDA-MB-231 to acquire great proliferative and migrative power assisting it to heal the manufactured scratch in both cell lines as compared to control. Overall, our result point out that miR-98 acts as oncomiR in breast cancer cell lines.
Keywords
ER-positive cells; ER-negative cells; miRNA-98; angiogenesis; metastasis; oxidative stress
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