Molecular Characterization and Pathological Findings of Syngamus trachea in Captive White Storks in University of Ibadan Zoological Garden, Nigeria | ||
| Journal of Applied Veterinary Sciences | ||
| Article 3, Volume 10, Issue 2, April 2025, Pages 13-17 PDF (424.71 K) | ||
| Document Type: Case Study | ||
| DOI: 10.21608/javs.2025.346511.1493 | ||
| Authors | ||
| Olaifa O.S.1; Ogbonna N.F.2; Anifowose O.R.* 3; Jarikre T.A.4; Awobode H.5; Ola O.O.1; Ogunro B.N.6; Tijani M.O.1; Adebowale E.A.7; Ohore O.G.1; Taiwo V.O.1; Usman A.A.1; Adebiyi T.O.6; Alaka A.A.1 | ||
| 1Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria | ||
| 2Department of Veterinary Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria | ||
| 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria | ||
| 4Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria. | ||
| 5Department of Zoology, University of Ibadan, Nigeria | ||
| 6Veterinary Teaching Hospital, University of Ibadan, Nigeria | ||
| 7Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria | ||
| Abstract | ||
| This case report highlights the clinical presentation, postmortem findings, and diagnostic evaluation of syngamosis and severe dehydration in two white storks (Ciconia ciconia). The storks, a male and female, introduced into a zoo four months prior, succumbed to parasitic tracheitis and dehydration. Necropsy and histopathology examinations were conducted while molecular parasite identification was carried out by using species-specific primers. Parasites found were cleaned with isotonic NaCl solution, preserved in 70% ethanol, and examined under microscopes. Intestinal scrapings were used to assess parasite egg burden, and various organ tissue samples were collected and sent for histopathological examination, including the trachea, lung, heart, liver, kidney, proventriculus, ventriculus (gizzard), small and large intestines, and bursa of Fabricius. Histopathological analysis was performed through paraffin embedding and staining with hematoxylin and eosin. Necropsy revealed dehydration, cachexia, parasitic tracheitis, nephrosis, and generalized hemorrhages. Histopathological examination confirmed subacute tracheitis with goblet cell hyperplasia, pulmonary hemorrhages, and renal damage. Molecular analysis identified Syngamus trachea via amplification of the 18S rRNA gene. This case report sheds light on the clinical presentation and pathological observation in white storks diagnosed with syngamosis and severe dehydration. The identification of parasitic tracheitis underscores the importance of considering parasitic infections in avian mortality investigations. Dehydration should also be addressed as a significant factor in assessing the health of captive birds. | ||
| Keywords | ||
| Avian Mortality; Dehydration; Parasitic Tracheitis; Syngamosis; White Stork | ||
| References | ||
|
BETHONICO, TERRA, M. T., PACHECO, W. J., HARRISON, M., MCCREA, B. A., and HAUCK, R., 2021. A survey of coccidia and nematodes in pastured poultry in the state of Georgia. Avian diseases, 65(2), 250-256. https://doi.org/10.1637/aviandiseases-d-20-00120
CARTA, L, K., and LI, S., 2018. Improved 18S small subunit rDNA primers for problematic nematode amplification. J. Nematol. 50 (4): 533-542. Article https://doi.org/10.21307/jofnem-2018-051
COLE, A, R. 2001. Tracheal Worms. USGS. https://pubs.usgs.gov/publication/2000999
DEPLAZES, P., JOACHIM, A., MATHIS, A., STRUBE, C., TAUBERT, A., SAMSON-HIMMELSTJERNA, G., and ZAHNER, H., 2021. Parasitologie für die Tiermedizin. https://shop.thieme.de/Parasitologie-fuer-die-Tiermedizin/9783132421387
FERNANDO, M, A., and BARTA, J, R., 2008. Tracheal worms. Parasitic diseases of wild birds, 343-354. http://dx.doi.org/10.1002/9780813804620.ch19
JOVANI, R, T., and TELLA, J, L., 2004. Age-related environmental sensitivity and weather mediated nestling mortality in white storks Ciconia Ciconia. Ecography, 27 (2004), pp. 611-618. https://www.jstor.org/stable/3683463
MARTIN, K, A., JESUDOSS, J, R, J., SATO, Y., and BREWER, M, T., 2020. Syngamus asphyxiation in a captive ring-necked pheasant. Vet. Parasitol. Reg. Stud. Rep., 22 (2020), Article 100493
MATSUBAYASHI, M., TAKAMI, K., KINOSHITA, M., TSUCHIDA, S., USHIDA, K., SHIBAHARA, T., and SASAI, K., 2021. Morphological and molecular identification of Eimeria tetartooimia oocysts from a Japanese green pheasant (Galliformes; Phasianidae; Phasianus versicolor) at a zoo in Japan. Parasitology Research, 120, 2973-2979.
MICHALCZYK, M, SOKÓŁ, R, GESEK, M, MĄCZYŃSKI, M., and BĘDZŁOWICZ, D., 2020. Internal parasites and associated histopathological changes in deceased white storks from Poland. Belg. J. Zool., 150, pp. 71-80
MEISTER, S. L., WENKER, C., WYSS, F., ZÜHLKE, I., VEIGA, I. B., and BASSO, W. U., 2022. Syngamus trachea in free-ranging white stork (Ciconia ciconia) nestlings in Switzerland. International Journal for Parasitology: Parasites and Wildlife, 18, 76-81. https://doi.org/10.1016/j.ijppaw.2022.04.007
NEVAREZ, J, B., GAMBLE, K, C., and TULLY, JR, T, N., 2002. Syngamus trachea infection in two red-and-yellow barbets (Trachyphonus erythrocephalus). J. Avian Med. Surg., 16 (2002), pp. 31-33. https://www.jstor.org/stable/30133203
OLIAS, P, GRUBER, A, D., HAFEZ, H, M., LIERZ, M, SLESIONA, S, BROCK, M, and JACOBSEN, I, D., 2011. Molecular epidemiology and virulence assessment of Aspergillus fumigatus isolates from white stork chicks and their environment. Vet. Microbiol., 148 pp. 348-355. https://doi.org/10.1016/j.vetmic.2010.08.029
TAMURA, K., STECHER, G., and KUMAR, S., 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol. Biol. Evo. 38(7), 3022–3027. https://doi.org/10.1093/molbev/msab120
ROBERTS, R.J. 2004. Fish Pathology. 3rd Edn., W.B. Saunders, USA. | ||
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