Protective impact of Hesperidin in hyperthyroid-induced Cardiomyopathy in rats

Khodir, Suzan A; Abd El-aziz, Noha; Emam, Reem M; Elmalky, Heba Ashraf; Abdalraouf, Mai M; Shahien, Mohamed Awad; Ahmed, Reem Mohammed; Abd-Elhamid, Tarek H; Abd Elmaksoud, Rasha Arafa; Elmorshdy, Shorouk E. M.

doi:10.21608/muj.2025.366265.1211

	Protective impact of Hesperidin in hyperthyroid-induced Cardiomyopathy in rats
Medicine Updates
Volume 23, Issue 23, October 2025, Page 30-43 PDF (1.27 MB)
Document Type: Original Article
DOI: 10.21608/muj.2025.366265.1211
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Authors
Suzan A Khodir ¹; Noha Abd El-aziz²; Reem M Emam³; Heba Ashraf Elmalky⁴; Mai M Abdalraouf⁵; Mohamed Awad Shahien⁶; Reem Mohammed Ahmed⁷; Tarek H Abd-Elhamid⁸; Rasha Arafa Abd Elmaksoud⁹; Shorouk E. M. Elmorshdy³
¹Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
²Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
³Medical Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
⁴Endocrinology unit, Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁵Cardiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁶Clinical Pharmacology Department, Faculty of medicine, Damitta University, Egypt
⁷Medical Biochemistry and Molecular Biology Department Faculty of Medicine (Girls), Al-Azhar University
⁸Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt. Histology, Faculty of Medicine, Aqaba Medical Sciences University, Aqaba, 77110, Jordan
⁹Pathology Department, Faculty of Medicine, PortSaid University, Egypt.
Abstract
Background: Higher risk of cardiovascular problems is linked to hyperthyroidism. The citrus bioflavonoid hesperidin (HSP) has cardioprotective, anti-inflammatory, and antioxidant impacts. Aim of the study: to illustrate the possible underlying processes and cardioprotective impact of HSP in cardiomyopathy caused by hyperthyroidism. Material and methods: Thirty male albino rats were split into three groups: Hyperthyroid, Hyperthyroid+HSP, and control (10/group). After four weeks rats were subjected to ECG evaluation, LVW/ tibial length, Serum TSH, Serum total T3, T4, Serum LDH, Serum CK-MB, Cardiac MDA, Cardiac SOD, Cardiac TNF-α, Cardiac IL-6, Cardiac genes expression of ANF, Caspase-3 and NF-kB evaluation. Furthermore, cardiac histological and immunohistochemical analyses were performed. Results: There was dramatically elevated heart rate, LVW/ tibial length, serum tT3, serum tT4, serum LDH, serum CK-MB, cardiac MDA, cardiac TNF-α, cardiac IL-6, cardiac ANF gene expression, cardiac Caspase-3 gene expression and cardiac NF-kB gene expression alongside a marked reduction in duration of PR interval and QT interval, serum TSH and cardiac SOD of hyperthyroid group compared to control. HSP dramatically ameliorated hyperthyroid-induced cardiac alterations generated by hyperthyroidism. Conclusion: In hyperthyroid-induced cardiomyopathy, HSP has a cardioprotective effect by enhancing ANF levels and regulating the heart's anti-inflammatory, anti-apoptotic, and antioxidant mechanisms.
Keywords
ANF; Cardiomyopathy; Hesperidin; Hyperthyroid; NF-kB


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