Phytochemical Profiling and Anticholinesterase Activity of Alpinia zerumbet: A Potential Source of Natural Therapeutics

Shahat, Esraa A.; Ayoub, Iriny M; Bakr, Riham O.; Gad, Haidy A.; Eldahshan, Omayma A.; Singab, Abdel-Nasser B.

doi:10.21608/aps.2024.322990.1198

	Phytochemical Profiling and Anticholinesterase Activity of Alpinia zerumbet: A Potential Source of Natural Therapeutics
Archives of Pharmaceutical Sciences Ain Shams University
Article 2, Volume 9, Issue 1, June 2025, Page 21-35 PDF (966.25 K)
Document Type: Original Article
DOI: 10.21608/aps.2024.322990.1198
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Authors
Esraa A. Shahat ¹; Iriny M Ayoub²; Riham O. Bakr¹; Haidy A. Gad²^{, 3}; Omayma A. Eldahshan ²^{, 4}; Abdel-Nasser B. Singab²^{, 4}
¹Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt
²Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt
³Department of Pharmacognosy, Faculty of Pharmacy, King Salman International University. South Sinai, Egypt
⁴Centre for Drug Discovery Research and Development, Ain Shams University, Cairo 11566, Egypt
Abstract
Alpinia zerumbet is an economically important species that belongs to the family Zingiberaceae. It is widely used in traditional medicines and nutraceuticals. This study explores the phytochemical constituents of the extracts of both leaves and rhizomes of A. zerumbet together with their in-vitro anticholinesterase effect. Using the LC/MS-MS technique, both leaves and rhizomes of A. zerumbet were analyzed leading to the annotation of 24 constituents in the methanolic extract of the leaves and 23 constituents in the rhizomes extract encompassing various classes including phenolic acids, flavonoids, kavalactones, diarylheptanoids, gingerols and terpenoids. Both extracts demonstrated promising anticholinesterase activity, with the rhizomes exhibiting a stronger effect (IC₅₀ = 2.229 ± 0.077 µg/mL) compared to the leaves (IC₅₀ = 3.573 ± 0.123 µg/mL). Tacrine served as the standard for anticholinesterase activity, with an IC₅₀= 0.428 ± 0.015 µg/mL. The results suggest that A. zerumbet, particularly its rhizomes, holds potential as a natural source of anticholinesterase agents, warranting further investigation for therapeutic applications.
Keywords
Alpinia zerumbet; leaves; rhizomes; anticholinesterase; LC-MS-MS; diarylheptanoid; kavalactones; gingerols


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