Effect of Purple Sweet Potato Leaf Extract Supplementation on the Histopathology of Gills, Liver, and Kidneys in Neolissochilus soroides Exposed to Ammonia as a Stressor | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 59, Volume 29, Issue 2, March and April 2025, Page 953-965 PDF (803.69 K) | ||||
| DOI: 10.21608/ejabf.2025.418367 | ||||
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| Author | ||||
| Suciyono et al. | ||||
| Abstract | ||||
| This study investigated the potential effect of purple sweet potato (Ipomoea batatas) leaf extract as an antioxidant on the histopathological changes in the gills, liver, and kidneys of Neolissochilus soroides exposed to NH4Cl. Over a 40-day supplementation period, four doses of the extract (0, 2.5, 5, and 7.5%) were administered with five replicates per dose. Following the supplementation, the fish were exposed to a 48-hour stressor of NH4Cl at 10ppm. Histopathological observations were conducted at three time points: before rearing, after 40 days of supplementation, and after 48 hours of NH4Cl exposure. The results revealed that purple sweet potato leaf extract supplementation significantly (P< 0.05) increased the number of normal cells in the liver and kidneys compared to the control group. The 5 and 7.5% doses showed no significant difference from each other but were significantly more effective than the 2.5% dose and the control. Furthermore, both the 5 and 7.5% doses significantly reduced cellular damage, such as necrosis, in the target organs induced by NH4Cl exposure compared to the control. These findings suggest that higher doses of Ipomoea batatas leaf extract increase normal cell in the liver and kidneys while mitigating cellular damage caused by NH4Cl stress. In conclusion, supplementation with purple sweet potato leaf extract provides a protective effect against organ damage in N. soroides under NH4Cl-induced stress, with higher doses showing the most significant beneficial outcomes in terms of cell preservation and damage reduction. | ||||
| Keywords | ||||
| Neolissochilus soroides; Ipomoea batatas; Antioxidant; Stressor test; Histophatology | ||||
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