Metrnl impact in streptozotocin induced diabetic nephropathy in rats, targeting Nrf2/HO-1 pathway | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Volume 45, Issue 2, April 2025, Page 258-268 PDF (716.23 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2025.349279.1195 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Suzan A Khodir   1; Amany Wahb2; Mohamed Ali Ibrahim3; Noha Abd El-aziz4; Amany Elfakhrany5; Haitham M. Sewilam6; Noha Shawky7; Shaimaa K Dawa8; Radwa Hassan Ali 9
| ||||
| 1Medical physiology department, faculty of Medicine, Menoufia University | ||||
| 2Medical Biochemistry and Molecular Biology Department, Faculty of medicine, Helwan University, Egypt | ||||
| 3Internal Medicine Department, Faculty of Medicine, Menoufia University Shebin El-Kom, Menoufia, Egypt | ||||
| 4Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt | ||||
| 5Clinical Pharmacology Department, Faculty of medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt. | ||||
| 6Department of Histology, Faculty of Medicine, Helwan University, Helwan, Egypt | ||||
| 7Medical Physiology Department, Faculty of Medicine, Helwan University, Egypt | ||||
| 8Pathology Department - Faculty of Medicine, Banha University, Egypt | ||||
| 9Medical Physiology Department, Faculty of Medicine, Ain Shams University,Cairo Egypt Faculty of Medicine, Armed Forces College of Medicine (AFCM), Cairo, Egypt | ||||
| Abstract | ||||
| Introduction: The most prevalent complications in diabetes mellitus is diabetic nephropathy (DN). Metrnl's role in endocrine metabolism has been well investigated. It has been demonstrated to contribute to the healing of inflammatory injury.Objective: to assess the potential underlying processes and the protective impact of Metrnl in DN caused by STZ Materials and Methods: Thirty adult male albino rats were split into: control, DN, and DN+Metrnl (10/group). Urine albumin, serum glucose, serum glycosylated Hb A1c, serum urea, serum creatinine, serum cystatin C, creatinine clearance, renal MDA, renal GSH, renal TNF-α, renal IL-10, and renal gene expression of HO-1 and Nrf2 were assessed. Additionally, immunohistochemical and renal histopathology studies were carried out.Results The DN group had significantly higher serum levels of glucose, glycosylated Hb A1c, urea, creatinine, cystatin C, urinary albumin, renal MDA, and renal TNF-α than the control group, but they also had significantly lower renal GSH, renal IL-10, creatinine clearance values, and Nrf2 and HO-1 gene expression. Furthermore, NF-kB and Bax immunoreaction were upregulated in the DN compared to control. The STZ-induced DN alterations significantly improved in the DN+Metrnl group.Conclusion: Metrnl offers substantial protection against STZ-induced DN through anti-oxidant and anti-inflammatory pathways, apoptosis inhibition, and the up-regulation of Nrf2 and HO-1 renal gene expressions. | ||||
| Keywords | ||||
| Bax Diabetic Nephropathy; HO-1 Metrnl; NF-kB Nrf2 | ||||
|
Statistics Article View: 86 PDF Download: 45 |
||||
