Baicalein mitigates Indomethacin-Induced Gastric Ulcer in Rats: Involvement of the PI3K/Akt/NF-kB pathway

Khodir, Suzan A; Wahb, Amany; Arafa, Mostafa Makram; Abd El-aziz, Noha; Shahien, Mohamed Awad; Safwat, Marwa Mohamed; Ali, Radwa Hassan; Abdel Wahab, Kariman Kariman Hussein; Shawky, Noha

doi:10.21608/besps.2025.349392.1196

	Baicalein mitigates Indomethacin-Induced Gastric Ulcer in Rats: Involvement of the PI3K/Akt/NF-kB pathway
Bulletin of Egyptian Society for Physiological Sciences
Volume 45, Issue 2, April 2025, Page 269-281 PDF (856.24 K)
Document Type: Original Article
DOI: 10.21608/besps.2025.349392.1196
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Authors
Suzan A Khodir ¹; Amany Wahb²; Mostafa Makram Arafa³; Noha Abd El-aziz⁴; Mohamed Awad Shahien⁵; Marwa Mohamed Safwat⁶; Radwa Hassan Ali ⁷; Kariman Kariman Hussein Abdel Wahab⁸; Noha Shawky⁹
¹Medical physiology department, faculty of Medicine, Menoufia University
²Medical Biochemistry and Molecular Biology Department, Faculty of medicine, Helwan University, Egypt
³Internal Medicine Department, Faculty of Medicine, Menoufia University Shebin El-Kom, Menoufia, Egypt
⁴Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
⁵Clinical Pharmacology Department, Faculty of medicine, Damitta University, Egypt
⁶Human Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Ain Shams, Cairo, Egypt Anatomy Department, IbnSina National College for Medical Studies, Jeddah.
⁷Medical Physiology Department, Faculty of Medicine, Ain Shams University,Cairo Egypt Faculty of Medicine, Armed Forces College of Medicine (AFCM), Cairo, Egypt
⁸Pathology Department, Faculty of Medicine, Helwan University, Egypt.
⁹Medical Physiology Department, Faculty of Medicine, Helwan University, Egypt
Abstract
Background: Peptic ulcer is a major health concern. Compared to other NSAIDs, indomethacin (IND) typically has a higher ulcerogenic potential. As a flavonoid, baicalein (Baic) has anti-inflammatory and antioxidant qualities. Objective: to evaluate the gastroprotective impact of Baic on stomach ulcers caused by IND and to identify any possible underlying processes. Material and Methods: Thirty male rats were split into three: control, Ulcer, Ulcer+ Baic (10/group). Gastric ulcer index values, gastric PH, gastric PGE2, gastric MDA, gastric SOD, gastric TNF-α, gastric IL-1β, and gastric genes expression of NF-kB, PI3K, and Akt were evaluated. In addition to histolopathological and COX2 immunohistochemical evaluation of gastric tissue were performed. Results: The Ulcer group showed substantially higher gastric ulcer index values, gastric MDA, gastric TNF-α, gastric IL-1β, and gastric gene expression of NF-kB and gastric COX2 immunoreaction with dramatically decline in gastric PH values, gastric SOD, gastric PGE2 and gastric genes expression of PI3K and Akt compared to the control. Baic dramatically improved IND-induced changes in gastric tissue. Conclusion: Baic has gastroprotective benefits in rats with IND-induced ulcers via modifying the Akt/Pi3k/NF-kB signaling pathway and by having anti-inflammatory and antioxidant qualities.
Keywords
Akt Baicalein; COX2 Gastric Ulcer; Indomethacin; NF-kB; PI3K


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