The Potential Protective Versus Therapeutic Role of Resveratrol in Lung Toxicity Induced by ZnO Nanoparticles in Adult Male Albino Rat: A Histological, Immunohistochemical and Biochemical Study.

Mohmed, Shereen Abdel Fattah; Ibrahim, Mohamed Emad El-din; Elwi, Heba Mostafa; Mekawy, Dina Mohmed; Ouais, Ghada Nady; Abdallah, Mashael Adel; El-Nahla, Mai Mohamed

doi:10.21608/ejh.2025.353969.2205

	The Potential Protective Versus Therapeutic Role of Resveratrol in Lung Toxicity Induced by ZnO Nanoparticles in Adult Male Albino Rat: A Histological, Immunohistochemical and Biochemical Study.
Egyptian Journal of Histology
Articles in Press, Accepted Manuscript, Available Online from 23 March 2025
Document Type: Original Article
DOI: 10.21608/ejh.2025.353969.2205
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Authors
Shereen Abdel Fattah Mohmed¹; Mohamed Emad El-din Ibrahim²; Heba Mostafa Elwi³; Dina Mohmed Mekawy⁴; Ghada Nady Ouais ⁵; Mashael Adel Abdallah⁶; Mai Mohamed El-Nahla⁷
¹Professor of Anatomy and embryology ,faculty of medicine Cairo university
²Professor of Anatomy and Embryology Faculty of Medicine, Cairo University.
³Lecturer of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
⁴Lecturer of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
⁵lecturer of Anatomy and embryology faculty of medicine Cairo university, Giza, Egypt
⁶Assistant Lecturer of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt.
⁷Lecturer of Anatomy and Embryology, Kasr Alainy ,Cairo University
Abstract
Zinc oxide nanoparticles (ZnO NPs) are widely consumed orally in different applications, food additives, drug and gene delivery systems within the field of medicine. However, the safety of these nanoparticles largely depends on factors such as the duration of exposure, the dose, or the concentration to which an individual is subjected. The lung tissue is liable to damage due to environmental and non-environmental toxic substances. Consequently, this research aimed to check out the potential toxic effects of orally administered ZnO NPs on a critical organ, namely the lungs, while also exploring the possible protective or therapeutic effect of resveratrol in mitigating these harmful effects. Fifty albino rats were divided into five groups: control, sham, toxicity, protective resveratrol and therapeutic resveratrol. LM examination, immune-histochemical; Bcl2, Bax, caspase-3 and TNF-alpha, histo-morphometric assessment, MDA, SOD, GPx and NO were examined. Western blotting for IL1 beta and IL4 proteins in addition to gene expression of HO-1 were also evaluated. Administration of resveratrol; whether protective or therapeutic; significantly reduced lung parenchymatous destruction, interstitial collagen fibers, Bax, Caspase 3, TNF-alpha expression levels, MDA and NO tissue levels. Whereas area percentage of Bcl2, SOD and GPx tissue levels were increased significantly compared to that of ZnO NPs toxicity groups. Protective resveratrol displayed better results than therapeutic one in ameliorating lung toxicity. These findings implied that resveratrol alleviates the histolopathological and biochemical effects of ZnO NPs-induced lung toxicity, furthermore, it enhanced the pro-apoptotic and antioxidant parameters which are triggered in toxicity groups. It is recommended to study the prophylactic role of resveratrol and the maximum safe dose and duration of use regarding oral ZnO NPs.
Keywords
Key words: ZnO NPs; Lung toxicity; resveratrol


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