Investigation of TYMS (rs 2853542) polymorphism and Cytomegalovirus in patients with Acute Lymphoblastic Leukemia
Hmeed, E., Mtashar, B., Ghiath, Y., Al-Alwany, S., Hussein, M., Faraj, Y. (2025). Investigation of TYMS (rs 2853542) polymorphism and Cytomegalovirus in patients with Acute Lymphoblastic Leukemia. EKB Journal Management System, 11(1), 233-242. doi: 10.21608/jbaar.2025.419350
Elaf Zuhair Hmeed; Baan Abdulatif Mtashar; Yusra Ghiath; Shakir H. Mohammed Al-Alwany; Mohanad Salam Hussein; Yusur Falah Faraj. "Investigation of TYMS (rs 2853542) polymorphism and Cytomegalovirus in patients with Acute Lymphoblastic Leukemia". EKB Journal Management System, 11, 1, 2025, 233-242. doi: 10.21608/jbaar.2025.419350
Hmeed, E., Mtashar, B., Ghiath, Y., Al-Alwany, S., Hussein, M., Faraj, Y. (2025). 'Investigation of TYMS (rs 2853542) polymorphism and Cytomegalovirus in patients with Acute Lymphoblastic Leukemia', EKB Journal Management System, 11(1), pp. 233-242. doi: 10.21608/jbaar.2025.419350
Hmeed, E., Mtashar, B., Ghiath, Y., Al-Alwany, S., Hussein, M., Faraj, Y. Investigation of TYMS (rs 2853542) polymorphism and Cytomegalovirus in patients with Acute Lymphoblastic Leukemia. EKB Journal Management System, 2025; 11(1): 233-242. doi: 10.21608/jbaar.2025.419350

Investigation of TYMS (rs 2853542) polymorphism and Cytomegalovirus in patients with Acute Lymphoblastic Leukemia

Journal of Bioscience and Applied Research
Volume 11, Issue 1, March 2025, Page 233-242  XML PDF (992.27 K)
Document Type: Original Article
DOI: 10.21608/jbaar.2025.419350
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Authors
Elaf Zuhair Hmeed email 1; Baan Abdulatif Mtashar2; Yusra Ghiath1; Shakir H. Mohammed Al-Alwany3; Mohanad Salam Hussein1; Yusur Falah Faraj1
1National Center of Hematology, Mustansiriyah University, Baghdad, Iraq
2Microbiology Department, College of Medicine, Mustansiriyah University, Iraq
3Department of Biology College of Science, Babylon University, Iraq
Abstract
Background: Acute Lymphoblastic Leukemia (ALL) is a type of cancer of blood and bone marrow. It is a fast-growing cancer that predominantly affects the lymphoid lineage of white blood cells, leading to the overproduction of abnormal lymphoblasts. It is the most frequently diagnosed form of leukemia in children, though it can also occur in adults. Objective: This study aimed to determine the association between TYMS (rs 2853542) gene polymorphism and the percentage of CMV in patients with ALL. Patients and methods: A case-control study was carried out on one hundred twenty blood specimens, including sixty patients referred to the national center of hematology/al Mustansiriyah University and diagnosed with Acute lymphoid leukemia and sixty specimens collected from persons as a healthy control group, from October 2023 to June 2024. Sequencing was used to identify TYMS (rs 2853542) gene polymorphism and the genome of Cytomegalovirus by using conventional PCR. Results: Out of 120 individuals, 60 patients diagnosed as ALL with a mean age (15.9±11.4) years and 60 healthy control group with a mean age (17.8±12.3) year. The difference in the frequency of the TYMS (rs 2853542) genotype distribution between patients and control groups was statistically significant. PCR testing for CMV revealed positive results in 8.3% (5 out of 60 cases) of ALL patients. While 3.3% (2 out of 60 cases) have positive DNA-CMV genome in AHC group, so there is non-significant value between these groups, High significance value was found between TYMS (rs 2853542) SNP and CMV- DNA in patients with ALL.
Keywords
TYMS (rs 2853542) gene; Acute Lymphoid Leukemia; PCR; sequencing
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