Comparsion of Antiparasitic Effects of Allium sativum and Triclabendazole on Eggs and Miracidium of Fasciola hepatica In-vitro | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Volume 34, Issue 2, April 2025, Page 409-414 PDF (409.79 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2025.362891.1494 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
lubna AQ al-ibrahimi   ; Ikhlas A, Marhoon; Marwa S. Alwan
| ||||
| Department of Biology, College of Education, University of Al-Qadisiyah, Iraq | ||||
| Abstract | ||||
| Background: Fascioliasis is a parasitic disease of animals (in cattle and sheep, but also in pigs, donkeys, etc.) that can be transmitted to humans or other definitive host. The infection is acquired by eating aquatic or semi- aquatic -plants that have the metacercaria of the worm attached to their stem or leaves, raw or poorly cooked. Eggs derived from their uteri were also appendaged. Diagnostic morphological traits of F. hepatica eggs. Three dimensional models of F. hepatica eggs were created to demonstrate the eggs features. Since fecal examination remains goldI standard in diagnosing fasciolosis in humans and animals worldwide, our findings may contribute to improved* diagnostics. Fasciola is a type of leaf shaped parasitic worms and Anti parasitic effects of Allium sativum and Triclabendazole on the eggs and Miracidium of Fasciola hepatica were done in vitro. Experiments were done with different concentrations of garlic alcoholic extract after the complete hatching of Fasciola eggs and the release of miracidium. Fasciola eggs were incubated for two weeks in suitable laboratory conditions to stimulate hatching. Objectives: Fascioliasis is a zoonotic and food-borne parasitic diseases that caused by Fasciola hepatica. Garlic (Allium sativum) is an important and valuable medical plant which is compatibie due to its medical properties and effects. Allium sativum is used as a liver protector, anti-inflammatory, anti-bacterial and anti-parasitic agent. Methodology: Anti parasitic effects of Allium sativum and Triclabendazole on the eggs and Miracidium of Fasciola hepatica were done invitro. Experiments were done with different concentrations of garlic alcoholic extract after the complete hatching of Fasciola eggs and the release of miracidium. Fasciola eggs were incubated for two weeks in laboratory conditions under suitable pH, light and temperature conditions to stimulate hatching. Then 10 microliters of non-chlorinated water containing 20 miracidia were added to the wells and 50 microliters of each extract at the rate of 2, 5, and 10μg/ml were added to the wells. We used dechlorinated water and Triclabendazole as negative and positive control groups. Results: The results showed that the hydroalcoholic extract of Allium sativum reduced the survival time and also the movement speed of Miracidium fasciola and finally stopped it. The concentration of 10 µg/ml of hydroalcoholic extract of Allium sativum destroyed Miracidia in 107 ± 2 seconds (P < 0.001). But at a concentration of 2 μg/ml, this time was 493 ± 2 seconds (P < 0.001). At a concentration of 10 µg/ml, the speed of miracidia reaching 0.07 ± 0.02 (mm/s) was determined (P<0.001). Conclusion: The use of garlic plant extract (Allium sativum) by inhibiting the developmentation of Fasciola hepatica eggs interrupts the life cycle of this parasite and can reduce the cases of contamination among livestock and humans. On the other hand, due to the fact that this extract is a natural product, it seems to have minimal negative effects on the ecosystem. | ||||
| Keywords | ||||
| In-vitro; Allium sativum; Triclabendazole; Miracidium; Fasciola hepatica | ||||
|
Statistics Article View: 140 PDF Download: 79 |
||||
