Effect of Green Silver Nanoparticles Combined with Moringa oleifera leaf Extract on the liver and Pancreas in Streptozotocin-Induced Diabetic Rats

Al Khuzaee, Mohammed F.; El Hamidy, Salim M.; El Nobey, Muhamed A.; Shaikh Omar, Abdulkader M.; Al Hunduwan, Khaled; Al Harthi, Abdulelah A.

doi:10.21608/eajbsz.2025.419842

	Effect of Green Silver Nanoparticles Combined with Moringa oleifera leaf Extract on the liver and Pancreas in Streptozotocin-Induced Diabetic Rats
Egyptian Academic Journal of Biological Sciences, B. Zoology
Volume 17, Issue 1, June 2025, Page 167-176 PDF (741.55 K)
Document Type: Original Article
DOI: 10.21608/eajbsz.2025.419842
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Authors
Mohammed F. Al Khuzaee¹; Salim M. El Hamidy ²; Muhamed A. El Nobey¹; Abdulkader M. Shaikh Omar ²; Khaled Al Hunduwan¹; Abdulelah A. Al Harthi¹
¹Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
²Princess Doctor Najla Bint Saud Al Saud Distinguished Research Centre for Biotechnology, King Abdulaziz University, Jeddah, Saudi Arabia.
Abstract
Diabetes mellitus (DM) is a global health challenge, with diabetic liver injury being a critical complication driven by oxidative stress, hyperglycemia, and inflammation. This study evaluates the synergistic therapeutic potential of green silver nanoparticles (Ag NPs) synthesized with Moringa oleifera leaf extract to mitigate liver dysfunction in streptozotocin (STZ)-induced diabetic rats. Ag NPs were characterized using transmission electron microscopy (TEM) to determine their morphology and size. Fifty adult albino rats were divided into five groups: Group I (control), Group II (diabetic), Group III (diabetic treated with Ag NPs), Group IV (diabetic treated with M. oleifera), and Group V (diabetic receiving combination treatment). Biochemical parameters, including glucose, HbA1c, insulin, ALT, AST, albumin, total protein, total bilirubin, GSH, SOD, CAT, and MDA, were assessed alongside histological examinations of the liver and pancreas. STZ-induced diabetic rats exhibited significant hyperglycaemia, elevated HbA1c levels, impaired liver function, and oxidative stress. Treatment with Ag NPs and M. oleifera leaf extract improved glucose metabolism, restored antioxidant defences, and normalized liver biomarkers. The combined treatment demonstrated superior efficacy, significantly reducing serum glucose, HbA1c, ALT, and AST levels while enhancing insulin, GSH, and SOD levels. Histological analysis confirmed the amelioration of STZ-induced hepatic and pancreatic structural damage. These findings underscore the therapeutic potential of green silver nanoparticles and M. oleifera leaf extract as a novel intervention targeting oxidative stress, inflammation, and liver dysfunction in diabetes.
Keywords
Diabetic Mellitus; Green Silver Nanoparticles; Liver Function; Moringa oleifera leaf; Streptozotocin; Pancreas


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