MicroRNA-16, Bcl-2, and MAPK: Potential Biomarkers for Oral Squamous Cell Carcinoma | ||||
| Ain Shams Dental Journal | ||||
| Volume 37, Issue 1, March 2025, Page 183-194 PDF (652.51 K) | ||||
| Document Type: Original articles | ||||
| DOI: 10.21608/asdj.2024.310949.1430 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Noha Mohamed Elfeky   1; Sabreen Gamal Khalil Amar 2; Abd El Rahman M Sharfeldeen 3; Enas Alaa Eldin Abd El-Aziz 4
| ||||
| 1Demonstrator at oral and maxillofacial pathology department, Faculty of dentistry Assiut university, Egypt | ||||
| 2Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt | ||||
| 3Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Egypt, Assistant Professor of oral pathology, Collage of dentistry, City University Ajman, Ajman, UAE | ||||
| 4Associate Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt. | ||||
| Abstract | ||||
| Aim: The objectives of the current study were to assess the level of miRNA‐16 expression in oral squamous cell carcinoma and its effect on cell apoptosis and proliferation following its suppression. Additionally, the study aimed to investigate the mechanisms that underlie the involvement of miRNA-16 and its target genes, Bcl-2 and, MAPK in the progression of OSCC. Materials and methods: The Oral Cavity Squamous Cell Carcinoma cell line (OECM-1) and Normal Human Tongue Fibroblasts (HOrF) were used in this in vitro study. The MTT assay was used to evaluate the cytotoxic effect following the cells' transfection with a miRNA-16 inhibitor. In both treated and untreated cells, the expression levels of the miRNA-16, Bcl-2, and MAPK genes were measured using SYBER green-based quantitative PCR. Tukey's multiple comparisons test and One-way analysis of variance (ANOVA) were used to analyze the data. Results: OECM-1 cells transfected with miRNA-16 inhibitor exhibited a significant increase in proliferation in comparison with the untreated and normal cells. Additionally, a significant increase in the expression of Bcl-2 and MAPK was associated with miRNA-16 inhibition compared to the untreated OECM-1 cells. Conclusion: The findings suggest that miRNA‐16 is a tumor suppressor miRNA that acts by cell proliferation inhibition and apoptosis induction in OSCC by directly targeting Bcl-2 and MAPK. This implies that miRNA‐16 could be a potential therapeutic target for OSCC. | ||||
| Keywords | ||||
| OSCC; MiRNA‐16; Bcl-2; MAPK | ||||
|
Statistics Article View: 256 PDF Download: 147 |
||||
