IN VITRO EVALUATION OF ORGANIC ACIDS' BIOFILMICIDAL AND ANTIMICROBIAL EFFICACY AGAINST SALMONELLA KENTUCKY AND PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM BROILER FARMS' DRINKING WATER | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 8, Volume 71, Issue 185, April 2025, Page 93-107 PDF (688.91 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2025.333622.1461 | ||||
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| Authors | ||||
ASMAA METWALLY ALI; SHIMAA A. E. NASR ; HANAN S. S KHALEFA 
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| Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. | ||||
| Abstract | ||||
| Water systems and drinking water are the primary sources of disease persistence and spread in chicken operations. Biofilm reduces sanitation programs' effectiveness, even when chemical disinfectants are used. In this study, we examined the ability of Salmonella and Pseudomonas species to form biofilms in broiler water systems. Ninety samples were collected from water pipelines and drinkers on six broiler farms. Seven Salmonella and 21 Pseudomonas isolates were detected. Regarding biofilm formation, salmonella species, and pseudomonas both developed biofilms to varying degrees. Moreover, S. Kentucky and P. aeruginosa were examined for their ability to form a biofilm on PVC surfaces. In addition, they were tested for the effectiveness of water acidification with organic acids (OAs) in eliminating planktonic cells and biofilms. In this experiment, PVC coupons were incubated with a single bacterial strain to stimulate the formation of biofilms. A 7-day test indicated that the strains could remain viable as planktonic cells and form biofilms on PVC coupons. One or more acids were used at a concentration of 1% to treat the biofilm. Propionic acid, citric acid, and OAs mixtures were able to eliminate all S. Kentucky and P. aeruginosa planktonic and biofilm counts after an hour of contact. In conclusion, OAs can be used to replace other chemical disinfectants in water applications and are considered antibacterial and biofilmicidal agents. Also, the use of two or more acids in combination is more effective than the use of one acid alone. | ||||
| Keywords | ||||
| Biofilm; Water Lines; Organic Acids; Salmonella; Pseudomonas | ||||
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