PROBIOTIC APPROACHES TO E. COLI IN MEAT AND CHICKEN PRODUCTS: PREVALENCE, RESISTANCE, AND VIRULENCE | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 12, Volume 71, Issue 185, April 2025, Page 151-174 PDF (969.95 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2025.340930.1502 | ||||
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| Authors | ||||
MOHAMED KORASHE DANDRAWY  1; TUFAHAH M.O. ATIYAHULLAH2; HASSAN MAHMOUD DIAB 3; MANAR M. ABDELALEEM4; AHMED SHABAN AHMED 5; NADY KHAIRY ELBARBARY6
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| 1Department of Food Hygiene and Control (Meat Hygiene), Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt. | ||||
| 2Department of Food Hygiene, Faculty of Veterinary Medicine, Omar ALmukhtar University, P.O. Box 919 ELBeida, Libya. | ||||
| 3Department of Animal and Poultry Health and Environment, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt. | ||||
| 4Department of Fish Health and Diseases, Faculty of Fish and Fisheries Technology, Aswan University, Aswan 81528, Egypt | ||||
| 5Department of Food Hygiene and Control (Milk Hygiene), Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt | ||||
| 6Department of Food Hygiene and Control (Meat Hygiene), Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt | ||||
| Abstract | ||||
| This study explores the prevalence of coliforms and pathogenic E. coli in chilled beef and poultry meat products sold in Qena City, Egypt, and assesses the efficacy of certain lactic acid bacteria (LAB) against these strains. A total of 320 samples, including various meat products, were examined using PCR, serological, and culture-based methods for E. coli identification. Antibacterial activities of LAB cell-free supernatant (CFS) were measured using the liquid-broth method. All samples showed coliform and fecal coliform contamination. E. coli counts above the acceptable limit (>0 cfu/g) were found in 80% of beef minced meat, 72.5% of beef sausage, 62.5% of beef kofta, 67.5% of beef burgers, 60% of chicken burgers, 75% of chicken liver, 80% of chicken nuggets, and 55% of chicken wings. Diverse diarrheagenic pathotypes, including UPEC, ETEC, EPEC, and STEC, were identified. Frequently detected serogroups included O158, O142, O63, O119, O55, O169, and O124. The hlyA and papC genes were present in 36.2 and 24.43% of isolates, respectively, while mcr1 and qnrB genes, coding for extended-spectrum β-lactamase (ESBL), were found in 5.43 and 5.88% of isolates. E. coli isolates exhibited high antimicrobial resistance and various resistance profiles, producing different biofilm phenotypes. LAB CFS significantly reduced E. coli CFU by eleven log10 orders. E. coli producing ESBL is commonly isolated from meat in Egypt, posing a significant public health risk due to poor sanitation in food processing facilities, leading to the transfer of resistant bacteria to humans. | ||||
| Keywords | ||||
| E. coli; meat products; Lactic acid bacteria (LAB); antibacterial activity | ||||
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