Antibacterial Effect of Natural Honey of known active components by GC-MS technique against Staphylococcus aureus | ||||
| Journal of Bioscience and Applied Research | ||||
| Article 15, Volume 11, Issue 2, June 2025, Page 533-548 PDF (1.53 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2025.358175.1148 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Rana S. Hasan1; Sawsan M. Alomari1; Wjdan Salim Qasim1; Ali M Saadi  2
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| 1Medical Laboratory Techniques Department / Mosul Medical Technical Institute / Northern Technical University, Mosul, Iraq | ||||
| 2Department of Medicinal Plant Technologies, Technical Agricultural College, Northern Technical University, Iraq | ||||
| Abstract | ||||
| Background: Staphylococcus aureus is a human pathogenic bacterium that colonizes the skin, oral cavity, upper respiratory tract, and gastrointestinal tract. It causes skin, soft tissue infections, and hospital-acquired infections. Aim: isolating Staph. aureus from skin infection and using honey as an alternative pathway to therapeutic therapeutic-resistant bacteria causing skin infection, as well as estimating the active compound by using the GC-MS Technique. Methods: (110) Clinical samples were collected from patients with skin infections through consultation clinics at Ibn Sina General Hospital of Mosul City/ Iraq, during the period from September 2024 until December 2024, by using conventional methods and the VITEK 2 system for identification. Result: The rate of isolation of Staph. aureus from skin infection was 76 (69.09%), but at the age group (11-30) years was 25% and (30-50) years was 40.7%, (51-70) years at 22.3% while at ages less than 10 years was 6.5% and ages more than 70 years was 5.2%. This bacterial species showed resistance against most of the 10 antibiotics under test using the disk diffusion method. Whole honey with its four dilutions (25,50,75, and 100%) gave an antibacterial effect against Staph. aureus growth. Gas Chromatography Mass Spectrometry (GC-MS) was used to investigate the biological effectiveness of honey and its (11) components, which have antibacterial effects. | ||||
| Keywords | ||||
| Staphylococcus aureus; skin infection; Antimicrobials agent; compound of honey; GC- MS Technique | ||||
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