Functional signatures of non-coding RNAs plasmacytoma variant translocation 1(PVT-1) and miR-135 in COVID-19
Elsayed, M., El-Mezayen, H., Shaker, O., Abdelfatah, Y., Abdo, S. (2025). Functional signatures of non-coding RNAs plasmacytoma variant translocation 1(PVT-1) and miR-135 in COVID-19. EKB Journal Management System, 68(12), 149-158. doi: 10.21608/ejchem.2025.356350.11231
Medhat M. Elsayed; Hatem A. El-Mezayen; Olfat G. Shaker; yasmine Abdelfatah; Sara M. Abdo. "Functional signatures of non-coding RNAs plasmacytoma variant translocation 1(PVT-1) and miR-135 in COVID-19". EKB Journal Management System, 68, 12, 2025, 149-158. doi: 10.21608/ejchem.2025.356350.11231
Elsayed, M., El-Mezayen, H., Shaker, O., Abdelfatah, Y., Abdo, S. (2025). 'Functional signatures of non-coding RNAs plasmacytoma variant translocation 1(PVT-1) and miR-135 in COVID-19', EKB Journal Management System, 68(12), pp. 149-158. doi: 10.21608/ejchem.2025.356350.11231
Elsayed, M., El-Mezayen, H., Shaker, O., Abdelfatah, Y., Abdo, S. Functional signatures of non-coding RNAs plasmacytoma variant translocation 1(PVT-1) and miR-135 in COVID-19. EKB Journal Management System, 2025; 68(12): 149-158. doi: 10.21608/ejchem.2025.356350.11231

Functional signatures of non-coding RNAs plasmacytoma variant translocation 1(PVT-1) and miR-135 in COVID-19

Egyptian Journal of Chemistry
Article 14, Volume 68, Issue 12, December 2025, Page 149-158  XML PDF (327.02 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2025.356350.11231
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Authors
Medhat M. Elsayed email orcid 1; Hatem A. El-Mezayenorcid 2; Olfat G. Shakerorcid 3; yasmine Abdelfatahorcid 4; Sara M. Abdo5
1Chemistry department, Faculty of science, Helwan university, cairo, Egypt.
2chemistry department, Faculty of science, Helwan University, Cairo , Egypt.
3Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
4Internal medicine, Faculty of Medicine, Cairo University
5Chemistry department, Faculty of Science Helwan University
Abstract
Background: Globally, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic had a major impact on the populations, and different responses implemented to control its spread. Limited and controversial data are available regarding the involvement of lncRNAs in COVID-19 pathogenesis. Objective: In this regard the current aimed to elucidate the relationship of Plasmacytoma variant translocation 1 (PVT-1) and MicroRNA 135 (miR-135) in COVID-19 and to assess the diagnostic performances of their expression levels to be employed as biomarkers. Furthermore, to investigate the reliable relations of these non-coding RNAs (ncRNAs) with other laboratory indices and clinical characteristics of COVID-19 patients. Methods: The study included 30 COVID-19 patients and 20 healthy controls. For all subjects, full medical investigations and laboratory analysis were performed. The expressions of candidate ncRNA; sPVT-1 and miRNA-135 were assessed by real-time quantitative polymerase chain reaction (RT-qPCR). Results: Among COVID-19 patients versus healthy individuals, PVT-1, expression levels were significantly up-regulated, while miRNA-135 expression was down-regulated. Besides miR-135 were negatively correlated. ROC curve analysis indicated diagnostic performances for both miR-135 (AUC = 0.96, p< 0.05) in COVID-19.
Conclusion: The PVT-1and miRNA-135 expression levels to be employed as biomarkers to detect severity of COVID-19.
Keywords
KEYWORDS: Non-coding RNAs plasmacytoma variant translocation 1(PVT-1); miR-135
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